Non-reductive scavenging of 1,1-diphenyl-2-picryihydrazyl (DPPH) by peroxyradical: A useful method for quantitative analysis of peroxyradical

被引：36
|
作者：
Nishizawa, M
Kohno, M
Nishimura, M
Kitagawa, A
Niwano, Y [1 ]
机构：
[1] Tohoku Univ, New Ind Creat Hatchery Ctr, Aoba Ku, Sendai, Miyagi 9808579, Japan
[2] Sunny Hlth Co Ltd, Bussiness Dev Div, Chuo Ku, Tokyo 1030028, Japan
来源：
CHEMICAL & PHARMACEUTICAL BULLETIN | 2005年 / 53卷 / 06期
关键词：
1,1-diphenyl-2-pierylhydrazyl (DPPH); non-reductive scavenging; peroxyradical; electron spin resonance (ESR);
D O I：
10.1248/cpb.53.714
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
A stable radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) has long been used as a convenient method for the antioxidant assay of biological materials such as cysteine, glutathione, ascorbic acid, tocopherol and polyhydroxy aromatic compounds (hydroquinone, pyrogallol, etc). In this study, non-reductive scavenging of DPPH was investigated by electron spin resonance (ESR) analyses for the purpose of developing a useful method for quantitative determination of peroxyradical. Since DPPH was degraded in the presence of peroxyradical derived from UV-irradiated benzoviperoxide and the peroxyradical-induced degradation of DPPH was inhibited by the addition of a spin trapping agent 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), it is concluded that DPPH is non-reductively scavenged by peroxyradical. Therefore, it is suggested that DPPH could be a useful agent for the quantitative measurement of peroxyradical.
引用
收藏
页码：714 / 716
页数：3
相关论文
共 23 条
  • [1] 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging ability of sake during storage
    Kitagaki, H
    Tsugawa, M
    [J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 87 (03) : 328 - 332
  • [2] Extracts and Constituents of Rubus chingii with 1,1-Diphenyl-2-picrylhydrazyl (DPPH) Free Radical Scavenging Activity
    Ding, Hsiou-Yu
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2011, 12 (06): : 3941 - 3949
  • [3] Quantitative spectrophotometric and EPR-determination of 1,1-diphenyl-2-picryl-hydrazyl (DPPH)
    N. D. Yordanov
    Aneta G. Christova
    [J]. Fresenius' Journal of Analytical Chemistry, 1997, 358 : 610 - 613
  • [4] Quantitative spectrophotometric and EPR-determination of 1,1-diphenyl-2-picryl-hydrazyl (DPPH)
    Yordanov, ND
    Christova, AG
    [J]. FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1997, 358 (05): : 610 - 613
  • [5] 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging by protein hydrolyzates from tuna cooking juice
    Jao, CL
    Ko, WC
    [J]. FISHERIES SCIENCE, 2002, 68 (02) : 430 - 435
  • [6] Observations on the estimation of scavenging activity of phenolic compounds using rapid 1,1-diphenyl-2-picrylhydrazyl (DPPH•) tests
    Nenadis, N
    Tsimidou, M
    [J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 2002, 79 (12) : 1191 - 1195
  • [7] DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity of flavonoids obtained from some medicinal plants
    Okawa, M
    Kinjo, J
    Nohara, T
    Ono, M
    [J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2001, 24 (10) : 1202 - 1205
  • [8] Prenylated Xanthones from the Bark of Garcinia xanthochymus and Their 1,1-Diphenyl-2-picrylhydrazyl (DPPH) Radical Scavenging Activities
    Chen, Yu
    Fan, Hua
    Yang, Guang-zhong
    Jiang, Yan
    Zhong, Fang-fang
    He, Hong-wu
    [J]. MOLECULES, 2010, 15 (10): : 7438 - 7449
  • [9] Comparative Study of 1,1-Diphenyl-2-picryl-hydrazyl Radical (DPPH•) Scavenging Capacity of the Antioxidant Xanthones Family
    Li, Xican
    [J]. CHEMISTRYSELECT, 2018, 3 (46): : 13081 - 13086
  • [10] Chemical constituents from the bark of Garcinia xanthochymus and their 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activities
    Zhong, Fang-Fang
    Chen, Yu
    Yang, Guang-Zhong
    [J]. HELVETICA CHIMICA ACTA, 2008, 91 (09) : 1695 - 1703
123