The Vangtat deposit is the major gold deposit in a new orogenic gold belt discovered and developed in southeastern Laos in the past two decades. Our study of the Vangtat orogenic gold deposit shows that it formed within a convergent margin along the western segment of the Poko suture zone, which marks the collision between the Indochina Terrane and the Kontum Massif. Shear structure and greenschist facies metamorphic rocks are the main hosts for gold mineralization in the Vangtat deposit. High-grade gold-bearing quartz-sulfide veins and wall rock hydrothermal alteration are genetically related, and alteration minerals consist of quartz, carbonate, graphite, white mica, chlorite, and pyrite. The study of fluid inclusions and wall rock alteration assemblages indicate that the Vangtat gold deposit was formed by an aqueous-carbonic, low salinity, reduced, weakly alkaline to near-neutral pH fluid, mainly composed of H2O, NaCl, CO2, CH4, N-2, H2S, H-2, O-2, Si, and K. Gold was predominantly carried as a sulfide complex. The precipitation of gold is related to wall rock sulfidation and the further reduction with the carbon-bearing host rock. The correlation between trapping temperature obtained from arsenopyrite geothermometry (360-395 degrees C) and homogenization temperature obtained from primary fluid inclusion microthermometry (240-250 degrees C), indicates a pressure and depth range for the Vangtat deposit of 380-420 MPa and 11-13 km, respectively. Gold appears as inclusions mainly in pyrite, and auriferous pyrite is homogeneous in textural, chemical, and sulfur isotopic composition. Auriferous pyrite delta S-34 values range from +4 to +6 parts per thousand, which is compatible with the delta S-34 composition of igneous rocks. Together with cobalt/nickel ratios from the Vangtat auriferous pyrite, which are significantly greater than one (avg. 6.3), we suggest that mafic-ultramafic rocks in the geologic basement of the Vangtat deposit are the plausible source of hydrothermal gold and sulfur. The formation of this orogenic gold deposit is associated with metamorphic dehydration and devolatilization that transported gold to the site of deposition.