Intracellular location of catalase-peroxidase hydroperoxidase I of Escherichia coli

被引：5
|
作者：
Hillar, A
Van Caeseele, L
Loewen, PC [1 ]
机构：
[1] Univ Manitoba, Dept Microbiol, Winnipeg, MB R3T 2N2, Canada
[2] Univ Manitoba, Dept Bot, Winnipeg, MB R3T 2N2, Canada
来源：
FEMS MICROBIOLOGY LETTERS | 1999年 / 170卷 / 02期
基金：
加拿大自然科学与工程研究理事会;
关键词：
catalase-peroxidase; cytoplasm; immunogold staining;
D O I：
10.1016/S0378-1097(98)00558-8
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The catalase-peroxidase hydroperoxidase I of Escherichia coli has been confirmed to be located in the cytoplasm using two independent methods. Catalase activity was found predominantly ( > 95%) in the cytoplasmic fraction following spheroplast formation. The cytoplasmic enzyme glucose-6-phosphate dehydrogenase and the periplasmic enzyme alkaline phosphatase were used as controls. The second method of immunogold staining for the enzyme in situ revealed an even distribution of the enzyme across the cell. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码：307 / 312
页数：6
相关论文
共 50 条
  • [1] GENETIC-MAPPING OF KATG, A LOCUS THAT AFFECTS SYNTHESIS OF THE BIFUNCTIONAL CATALASE-PEROXIDASE HYDROPEROXIDASE-I IN ESCHERICHIA-COLI
    LOEWEN, PC
    TRIGGS, BL
    GEORGE, CS
    HRABARCHUK, BE
    [J]. JOURNAL OF BACTERIOLOGY, 1985, 162 (02) : 661 - 667
  • [2] New strategies in production of thermophilic catalase-peroxidase AfKatG in Escherichia coli
    Struharnanska, E.
    Levarski, Z.
    Stuchlik, S.
    Birova, S.
    Zamocky, M.
    Turna, J.
    [J]. FEBS JOURNAL, 2017, 284 : 105 - 105
  • [3] REGULATION OF HYDROPEROXIDASE (CATALASE) EXPRESSION IN ESCHERICHIA-COLI
    SCHELLHORN, HE
    [J]. FEMS MICROBIOLOGY LETTERS, 1995, 131 (02) : 113 - 119
  • [4] Modulation of the activities of catalase-peroxidase HPI of Escherichia coli by site-directed mutagenesis
    Hillar, A
    Peters, B
    Pauls, R
    Loboda, A
    Zhang, HM
    Mauk, AG
    Loewen, PC
    [J]. BIOCHEMISTRY, 2000, 39 (19) : 5868 - 5875
  • [5] Structure of the C-terminal domain of the catalase-peroxidase KatG from Escherichia coli
    Carpena, X
    Melik-Adamyan, W
    Loewen, PC
    Fita, I
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 : 1824 - 1832
  • [6] The catalase-peroxidase of Synechococcus PCC 7942: Purification, nucleotide sequence analysis and expression in Escherichia coli
    Mutsuda, M
    Ishikawa, T
    Takeda, T
    Shigeoka, S
    [J]. BIOCHEMICAL JOURNAL, 1996, 316 : 251 - 257
  • [7] Purification and characterization of an intracellular catalase-peroxidase from Penicillium simplicissimum
    Fraaije, MW
    Roubroeks, HP
    Hagen, WR
    vanBerkel, WJH
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 235 (1-2): : 192 - 198
  • [8] Is Root Catalase a Bifunctional Catalase-Peroxidase?
    Chioti, Vasileia
    Zervoudakis, George
    [J]. ANTIOXIDANTS, 2017, 6 (02)
  • [9] Nucleotide sequence analysis, overexpression in Escherichia coli and kinetic characterization of Anacystis nidulans catalase-peroxidase
    Engleder, M
    Regelsberger, G
    Jakopitsch, C
    Furtmüller, PG
    Rüker, F
    Peschek, GA
    Obinger, C
    [J]. BIOCHIMIE, 2000, 82 (03) : 211 - 219
  • [10] THE CATALASE-PEROXIDASE OF MYCOBACTERIUM-INTRACELLULARE - NUCLEOTIDE-SEQUENCE ANALYSIS AND EXPRESSION IN ESCHERICHIA-COLI
    MORRIS, SL
    NAIR, J
    ROUSE, DA
    [J]. JOURNAL OF GENERAL MICROBIOLOGY, 1992, 138 : 2363 - 2370
12345