Influence of hsa-miR-6727-5p on the proliferation, apoptosis, invasion and migration of Caski, Hela and SiHa cervical cancer cells

Purpose: To study the influence of hsa-miR-6727-5p on the proliferation, apoptosis, invasion and migration of Caski, Hela and SiHa cervical cancer cells. Methods: The CCK8 test, flow cytometry, Transwell and scratch healing experiments were used to measure the influence of hsa-miR-6727-5p on the proliferation, apoptosis, invasion and migration of Caski, Hela and SiHa cells. Results: Compared with the negative control (empty vector) group, the hsa-miR-6727-5p expression in the cells transfected with hsa-miR-6727-5p mimics showed a significant increase (p<0.01). CCK8 results showed that the hsa-miR-6727-5p overexpression enhanced significantly the proliferation of cervical cancer cells. Annexin V FITC/PI staining cell results showed that hsa-miR-6727-5p overexpression strongly inhibited the cervical cancer cells apoptosis. Tranoverexpression significantly enhanced the invasion ability of cervical cancer cells. The scratch healing test suggested that, compared with the control group, the migration ability of cells transfected with hsa-miR-6727-5p mimics in the experimental group was stronger. Conclusion: We confirmed at the cellular level that hsa-miR-6727-5p promoted the proliferation, invasion and migration of cervical cancer cells and inhibited the apoptosis through the overexpression or inhibition of hsa-miR-6727-5p. These results indicated that hsamiR-6727-5p played a role in promoting the cervical cancer.