Isolation of trichogin, an antifungal protein from fresh fruiting bodies of the edible mushroom Tricholoma giganteum

被引：30
|
作者：
Guo, YX
Wang, HX
Ng, TB [1 ]
机构：
[1] City Univ Hong Kong, Fac Med, Dept Biochem, Shatin, Hong Kong, Peoples R China
[2] China Agr Univ, Coll Biol Sci, Dept Microbiol, Beijing, Peoples R China
[3] State Key Lab Agrobiotechnol, Beijing, Peoples R China
来源：
PEPTIDES | 2005年 / 26卷 / 04期
关键词：
antifungal protein; Fusarium oxysporum; reverse transcriptase; Tricholoma giganteum;
D O I：
10.1016/j.peptides.2004.11.009
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An antifungal protein was isolated from the mushroom Tricholoma giganteum var. golden blessings. The protocol included ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose, and gel filtration by fast protein liquid chromatography on Superdex 75. The antifungal protein, designated trichogin, was unadsorbed on DEAE-cellulose but was adsorbed on Affi-gel blue gel and CM-cellulose. It exhibited antifungal activity against Fusarium oxysporum, Mycosphaerella arachidicola and Physalospora piricola. Trichogin inhibited HIV-1 reverse transcriptase with an IC50 of 83 nM. (c) 2004 Published by Elsevier Inc.
引用
收藏
页码：575 / 580
页数：6
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