The benzoquinone-mediated electrochemical microbial biosensor for water biotoxicity assay

被引：37
|
作者：
Li, Jiuming [1 ,2 ]
Yu, Yuan [1 ]
Wang, Yuning [1 ,2 ]
Qian, Jun [1 ]
Zhi, Jinfang [1 ]
机构：
[1] Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Photochem Convers & Oproelect Mat, Beijing 100190, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100190, Peoples R China
来源：
ELECTROCHIMICA ACTA | 2013年 / 97卷
基金：
北京市自然科学基金;
关键词：
Mediated microbial biosensor; Biotoxicity; Benzoquinone; TOXICITY ASSESSMENT; HEAVY-METALS; GLUCOSE; FERRICYANIDE; ELECTRODE;
D O I：
10.1016/j.electacta.2013.02.071
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
A simple mediated microbial biosensor providing real-time monitoring of water quality and evaluation of biotoxicity was fabricated by entrapping Escherichia coli (E. coli) cells in gelatin on glassy carbon electrode with benzoquinone as the redox mediator. The biotoxicity assay was based on the respiratory activity of coli cells estimated by the oxidation current of microbially reduced benzoquinone. The neutrality and lipophilicity rendered benzoquinone better efficiency than ferricyanide in mediated microbial reactions. After the optimization of preparation conditions, the prepared microbial biosensors have measured several common toxicants with different concentrations. In addition, the biotoxicity of binary mixtures of heavy metals and wastewater were investigated. The fabricated biosensor exhibited good repeatability and stability in the biotoxicity measurements. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：52 / 57
页数：6
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