Excision of 8-oxoguanine from methylated CpG dinucleotides by human 8-oxoguanine DNA glycosylase

被引：16
|
作者：
Kasymov, Rustem D. [1 ]
Grin, Inga R. [1 ,2 ]
Endutkin, Anton V. [1 ]
Smirnov, Serge L. [3 ]
Ishchenko, Alexander A. [2 ]
Saparbaev, Murat K. [2 ]
Zharkov, Dmitry O. [1 ,4 ]
机构：
[1] SB RAS Inst Chem Biol & Fundamental Med, Novosibirsk 630090, Russia
[2] Univ Paris 11, Inst Cancerol Gustave Roussy, CNRS UMR8200, Grp Reparat ADN, Villejuif, France
[3] Western Washington Univ, Dept Chem, Bellingham, WA 98225 USA
[4] Novosibirsk State Univ, Fac Nat Sci, Dept Mol Biol, Novosibirsk 630090, Russia
来源：
FEBS LETTERS | 2013年 / 587卷 / 18期
基金：
俄罗斯基础研究基金会;
关键词：
DNA repair; Epigenetic methylation; 5-Methylcytosine; 8-Oxoguanine; OGG1; AP LYASE ACTIVITY; SUBSTRATE-SPECIFICITY; POLYMORPHIC VARIANT; REPAIR; PROTEIN; ENDONUCLEASE; STIMULATION; RECOGNITION; MECHANISM; PRODUCT;
D O I：
10.1016/j.febslet.2013.08.008
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
CpG dinucleotides are targets for epigenetic methylation, many of them bearing 5-methylcytosine (mCyt) in the human genome. Guanine in this context can be easily oxidized to 8-oxoguanine (oxoGua), which is repaired by 8-oxoguanine-DNA glycosylase (OGG1). We have studied how methylation affects the efficiency of oxoGua excision from damaged CpG dinucleotides. Methylation of the adjacent cytosine moderately decreased the oxoGua excision rate while methylation opposite oxoGua lowered the rate of product release. Cytosine methylation abolished stimulation of OGG1 by repair endonuclease APEX1. The OGG1 S326C polymorphic variant associated with lung cancer showed poorer base excision and lost sensitivity to the opposite-base methylation. The overall repair in the system reconstituted from purified proteins decreased for CpG with mCyt in the damaged strand. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
引用
收藏
页码：3129 / 3134
页数：6
相关论文
共 50 条
  • [1] Activation of Ras Signaling Pathway by 8-Oxoguanine DNA Glycosylase Bound to Its Excision Product, 8-Oxoguanine
    Boldogh, Istvan
    Hajas, Gyorgy
    Aguilera-Aguirre, Leopoldo
    Hegde, Muralidhar L.
    Radak, Zsolt
    Bacsi, Attila
    Sur, Sanjiv
    Hazra, Tapas K.
    Mitra, Sankar
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (25) : 20769 - 20773
  • [2] Sequence specificity of human 8-oxoguanine DNA glycosylase
    Hummel, Sara
    Hoss, Matthew
    Haushalter, Karl A.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2006, 231
  • [3] Recognition of DNA damages by human 8-oxoguanine DNA glycosylase
    Kuznetsov, N. A.
    Kuznetsova, A. A.
    Ishchenko, A. A.
    Saparbaev, M. K.
    Fedorova, O. S.
    FEBS JOURNAL, 2013, 280 : 151 - 152
  • [4] Recognition of DNA damages by human 8-oxoguanine DNA glycosylase
    Kuznetsov, N. A.
    Kuznetsova, A. A.
    Ishchenko, A. A.
    Saparbaev, M. K.
    Fedorova, O. S.
    FEBS JOURNAL, 2013, 280 : 72 - 72
  • [5] Characterization of an 8-Oxoguanine DNA Glycosylase from Methanococcus jannaschii
    Gogos, Arhonda
    Clarke, Neil D.
    Journal of Biological Chemistry, 274 (43): : 30447 - 30450
  • [6] Cloning and characterization of a mammalian 8-oxoguanine DNA glycosylase
    Rosenquist, TA
    Zharkov, DO
    Grollman, AP
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (14) : 7429 - 7434
  • [7] Characterization of an 8-oxoguanine DNA glycosylase from Methanococcus jannaschii
    Gogos, A
    Clarke, ND
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (43) : 30447 - 30450
  • [8] Expression of 8-oxoguanine Glycosylase in Human Fetal Membranes
    Menon, Ramkumar
    Polettini, Jossimara
    Syed, Tariq Ali
    Saade, George R.
    Boldogh, Istvan
    AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2014, 72 (01) : 75 - 84
  • [9] Potent and Selective Inhibitors of 8-Oxoguanine DNA Glycosylase
    Tahara, Yu-ki
    Auld, Douglas
    Ji, Debin
    Beharry, Andrew A.
    Kietrys, Anna M.
    Wilson, David L.
    Jimenez, Marta
    King, Daniel
    Nguyen, Zachary
    Kool, Eric T.
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2018, 140 (06) : 2105 - 2114
  • [10] Thermodynamic and kinetic basis for recognition and repair of 8-oxoguanine in DNA by human 8-oxoguanine-DNA glycosylase
    Kirpota, Oleg O.
    Endutkin, Anton V.
    Ponomarenko, Michail P.
    Ponomarenko, Petr M.
    Zharkov, Dmitry O.
    Nevinsky, Georgy A.
    NUCLEIC ACIDS RESEARCH, 2011, 39 (11) : 4836 - 4850
12345