Stable isotope fractionation during the methanogenic degradation of organic matter in the sediment of an acidic bog lake, Lake Grosse Fuchskuhle

被引:55
|
作者
Conrad, Ralf [1 ]
Claus, Peter [1 ]
Casper, Peter [2 ]
机构
[1] Max Planck Inst Terr Microbiol, D-35043 Marburg, Germany
[2] Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Limnol Stratified Lakes, Stechlin, Germany
关键词
ARCHAEAL COMMUNITY; METHANE PRODUCTION; BIOGENIC METHANE; METHYL-FLUORIDE; CARBON; MARINE; BIOGEOCHEMISTRY; FERMENTATION; RESPIRATION; INHIBITION;
D O I
10.4319/lo.2010.55.5.1932
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Lake Grosse Fuchskuhle is an acidic bog lake, which develops an anoxic hypolimnion during the summer season, so that sediment organic matter is degraded anaerobically to CH4 and CO2. The delta C-13 values of organic matter and its degradation products were used for determination of the mass balance, degradation path, and fractionation factors. Addition of methyl fluoride, an inhibitor of acetoclastic methanogenesis, increasingly inhibited CH4 production and resulted in accumulation of acetate (and a little propionate), which then was an additional end product of organic matter degradation. The delta C-13 of acetate-methyl group was slightly lower than that of organic matter, indicating a small fractionation (about 4 parts per thousand) during the fermentative production of acetate. Chemolithotrophic acetogenesis was low (< 9%). The fraction of CH4 produced from hydrogenotrophic methanogenesis was about 55-67%. After incubation for 20 h the average delta C-13 of the degradation products was similar to that of sediment organic matter, both in the absence and the presence of CH3F. The produced total inorganic carbon (TIC) was further converted to CH4 with apparent enrichment factors of 56-67 parts per thousand. The fraction of TIC that was actually converted to CH4 was calculated to be about 33% and 23% in the absence and presence of CH3F, respectively. These data are consistent with the assumption that about half of the organic matter was incompletely degraded, resulting in the production of only CO2 and H-2, afterward converted to CH4. The data further show that the reduction of CO2 to CH4 was partially inhibited in the presence of CH3F.
引用
收藏
页码:1932 / 1942
页数:11
相关论文
共 50 条
  • [1] Characterization of methanogenic Archaea and stable isotope fractionation during methane production in the profundal sediment of an oligotrophic lake (Lake Stechlin, Germany)
    Conrad, Ralf
    Chan, On-Chim
    Claus, Peter
    Casper, Peter
    [J]. LIMNOLOGY AND OCEANOGRAPHY, 2007, 52 (04) : 1393 - 1406
  • [2] Methanogenic archaeal community in the sediment of an artificially partitioned acidic bog lake
    Chan, OC
    Wolf, M
    Hepperle, D
    Casper, P
    [J]. FEMS MICROBIOLOGY ECOLOGY, 2002, 42 (01) : 119 - 129
  • [3] Novosphingobium aquaticum sp nov., isolated from the humic-matter-rich bog lake Grosse Fuchskuhle
    Glaeser, Stefanie P.
    Bolte, Kathrin
    Busse, Hans-Juergen
    Kaempfer, Peter
    Grossart, Hans-Peter
    Glaeser, Jens
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2013, 63 : 2630 - 2636
  • [4] Characterization of stable isotope fractionation during methane production in the sediment of a eutrophic lake, Lake Dagow, Germany
    Conrad, Ralf
    Claus, Peter
    Casper, Peter
    [J]. LIMNOLOGY AND OCEANOGRAPHY, 2009, 54 (02) : 457 - 471
  • [5] Organic matter preservation in the sediment of an acidic mining lake
    Laskov, C
    Amelung, W
    Peiffer, S
    [J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2002, 36 (20) : 4218 - 4223
  • [6] Carbon and nitrogen stable isotope fractionation in the sediment of Lake Bled (Slovenia)
    S. Lojen
    N. Ogrinc
    T. Dolenec
    [J]. Water, Air, and Soil Pollution, 1997, 99 : 315 - 323
  • [7] Carbon and Nitrogen Stable Isotope Fractionation in the Sediment of Lake Bled (Slovenia)
    S. Lojen
    N. Ogrinc
    T. Dolenec
    [J]. Water, Air, and Soil Pollution, 1997, 99 : 315 - 323
  • [8] Carbon and nitrogen stable isotope fractionation in the sediment of Lake Bled (Slovenia)
    Lojen, S
    Ogrinc, N
    Dolenec, T
    [J]. WATER AIR AND SOIL POLLUTION, 1997, 99 (1-4): : 315 - 323
  • [9] Organic matter degradation in Lake Baikal - A sediment trap study
    Schubert, Carsten J.
    Niggemann, Jutta
    Lomstein, Bente Aa.
    Sturm, Michael
    [J]. GEOCHIMICA ET COSMOCHIMICA ACTA, 2008, 72 (12) : A840 - A840
  • [10] Stable carbon isotope fractionation, carbon flux partitioning and priming effects in anoxic soils during methanogenic degradation of straw and soil organic matter
    Conrad, Ralf
    Klose, Melanie
    Yuan, Quan
    Lu, Yahai
    Chidthaisong, Amnat
    [J]. SOIL BIOLOGY & BIOCHEMISTRY, 2012, 49 : 193 - 199