A real-time polymerase chain reaction assay for the detection of Mycoplasma agalactiae

被引：4

作者：

Fitzmaurice, J. ^{[1
]}

Sewell, M. ^{[1
]}

King, C. M. ^{[1
]}

McDougall, S. ^{[2
]}

McDonald, W. L. ^{[1
]}

O'Keefe, J. S. ^{[1
]}

机构：

[1] Minist Agr & Forestry, Biosecur New Zealand, Invest & Diagnost Ctr, Upper Hutt, New Zealand

[2] Ctr Anim Hlth, Morrinsville, New Zealand

来源：

NEW ZEALAND VETERINARY JOURNAL | 2008年 / 56卷 / 05期

关键词：

Mycoplasma agalactiae; real-time PCR;

D O I：

10.1080/00480169.2008.36839

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

AIM: To develop a real-time PCR for the detection of Mycoplasma agalactiae, using PCR primers targeting the ma-mp81 gene. METHODS: A group of 15 M. agalactiae isolates, 21 other Mycoplasma spp. isolates and 21 other bacterial isolates was used in evaluation of the assay. RESULTS: All M. agalactiae isolates were detected by the assay and none of the non-target isolates was amplified. The analytical detection limit of the assay was 10 fg of purified genomic DNA and 10(4) cfu/ml milk inoculated with M. agalactiae. When applied to goat-milk samples collected from three herds free of M. agalactiae infection, the assay had a specificity of 100%. CONCLUSIONS: The assay would be useful in a diagnostic laboratory, providing specific, sensitive and rapid detection of M. agalactiae.

引用

页码：233 / 236

页数：4

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