Polymorphic phase behavior of fluorocarbon double-chain phosphocholines derived from diaminopropanol, serine and ethanolamine and long-term shelf stability of their liposomes

被引:9
|
作者
Clary, L
Verderone, G
Santaella, C
Vierling, P
Chang, P
机构
[1] UNIV NICE,CHIM BIOORGAN LAB,UPRESA 6001 CNRS,FAC SCI,F-06108 NICE 2,FRANCE
[2] UNIV PARIS 06,URA 671 CNRS,UNITE BIOL CELLULAIRE MARINE,F-06230 VILLEFRANCHE MER,FRANCE
基金
中国国家自然科学基金; 上海市自然科学基金;
关键词
perfluoroalkylated phospholipids; fluorinated liposomes; vesicles; membranes; polymorphism; shelf-stability;
D O I
10.1016/S0009-3084(97)02657-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This paper describes the morphological characterization, by freeze-fracture electron microscopy, and the thermotropic phase behavior, by differential scanning calorimetry, of aqueous dispersions of various fluorocarbon/fluorocarbon or mixed fluorocarbon/hydrocarbon double-chain 2,3- or 1,3-diamidopropano-, diamidoserino- or amidoethyl-phosphocholines (PC). These fluorinated PCs form lamellar phases and liposomes, as their ester or ether glycerol-based analogs. The effects on the thermodynamic parameters associated with these lamellar phases of several structural elements-chemical junction (secondary CONHR or tertiary CONRR), connecting unit (diamino-propanol, serine or ethanolamine), number and length of the perfluoroalkylated chains, length of the fluorinated tail and hydrocarbon-are discussed. These data are compared with those reported for fluorocarbon or hydrocarbon glycerophosphocholines and hydrocarbon diamidophosphocholines, and for sphingomyelins. Most of the liposomes formed from the fluorinated amido-connected PCs display a remarkable long-term shelf stability: they can be thermally sterilized and stored at room temperature for several months without any significant modification of their size and size distribution. (C) 1997 Elsevier Science Ireland Ltd.
引用
收藏
页码:21 / 35
页数:15
相关论文
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