Molecular characterization of two prunus necrotic ringspot virus isolates from Canada

被引：14
|
作者：
Cui, Hongguang [1 ,2 ]
Hong, Ni [2 ]
Wang, Guoping [2 ]
Wang, Aiming [1 ]
机构：
[1] Agr & Agri Food Canada, So Crop Protect & Food Res Ctr, London, ON N5V 4T3, Canada
[2] Huazhong Agr Univ, Natl Key Lab Agromicrobiol, Wuhan 430070, Hubei, Peoples R China
来源：
ARCHIVES OF VIROLOGY | 2012年 / 157卷 / 05期
基金：
加拿大自然科学与工程研究理事会;
关键词：
ALFALFA MOSAIC-VIRUS; COAT PROTEIN; GENETIC DIVERSITY; MOVEMENT; RNAS;
D O I：
10.1007/s00705-012-1247-5
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We determined the entire RNA1, 2 and 3 sequences of two prunus necrotic ringspot virus (PNRSV) isolates, Chr3 from cherry and Pch12 from peach, obtained from an orchard in the Niagara Fruit Belt, Canada. The RNA1, 2 and 3 of the two isolates share nucleotide sequence identities of 98.6%, 98.4% and 94.5%, respectively. Their RNA1- and 2-encoded amino acid sequences are about 98% identical to the corresponding sequences of a cherry isolate, CH57, the only other PNRSV isolate with complete RNA1 and 2 sequences available. Phylogenetic analysis of the coat protein and movement protein encoded by RNA3 of Pch12 and Chr3 and published PNRSV isolates indicated that Chr3 belongs to the PV96 group and Pch12 belongs to the PV32 group.
引用
收藏
页码：999 / 1001
页数：3
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