Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing

Raw camel milk samples were collected from three geo-graphical locations (south, north and middle Kuwait) during two seasons. Next generation sequencing of the V3-V4 re-gions of the 16S rRNA gene was used to analyze the bacte-rial community in camel milk. DNA was extracted from one hundred thirty-three samples, and libraries were prepared using custom fusion primers of the 16S rRNA gene and se-quenced on Illumina HiSeq 2500 platform. 16S rRNA gene sequences were aligned against the SILVA database SSU re-lease 138. The high-throughput sequencing data are avail-able at the NCBI database under the Bioproject PRJNA814013. This work describes camel milk's bacterial diversity among different geographical locations and seasons. The distribu-tion of alpha diversity measures among camel milk sample groups collected from different geographical locations and seasons is presented. A significant effect of these parame-ters on camel milk's bacterial diversity was shown. Linear discriminant analysis (LefSe) showed significant differentially abundant bacteria at the phylum, class, order, family and genus level among the three locations and seasons. LefSe identified a total of 83 and 40 differentially abundant gen-era in the different geographical locations and seasons, re-spectively. More details about the bacterial composition of raw camel milk at the phylum and genus level can be found in research article [1] . These data can be used to compare the diversity of milk bacterial community between different milk producing species and camels from different parts of the world. Besides, these findings will contribute to our un-derstanding of the camel microbiome structure and might be useful for designing an appropriate control program in the camel dairy herd. The data described in this article are avail-able in Mendeley Data [2].(c) 2022 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )