Digital image processing for rapid analysis of differentially expressed transcripts on high-density cDNA arrays

被引：7
|
作者：
Adryan, B [1 ]
Carlguth, V [1 ]
Decker, HJ [1 ]
机构：
[1] Univ Mainz, Med Clin 3, Dept Hematol Oncol, Lab Tumorgenet & Mol Cytogenet, D-55131 Mainz, Germany
来源：
BIOTECHNIQUES | 1999年 / 26卷 / 06期
关键词：
D O I：
10.2144/99266bc03
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Usage of filter arrays is becoming increasingly attractive for many research laboratories involved indetermination of gene-expression profiles. However analysis of numerous spots, representing genes or partial gene sequences (ESTs), is still tedious work involving the ordered analysis of vast amounts of numerical tabular data. We present a vapid and efficient method for the visual identification of differentially expressed targets on high-density cDNA filter arrays using standard laboratory equipment and standard software, which is available for free. The method we introduce provides an inexpensive alternative, and no changes in the experimental set up are required. Our results were verified by densitometric analyses performed with an established system.
引用
收藏
页码：1174 / +
页数：5
相关论文
共 50 条
  • [1] Enhanced analysis of differentially expressed transcripts on high-density cDNA arrays using digital image processing
    Rüdiger, S
    Brieger, J
    Weidt, E
    Adryan, B
    Winterpacht, A
    Brenner, W
    Zabel, B
    Huber, C
    Decker, HJ
    EUROPEAN JOURNAL OF HUMAN GENETICS, 1998, 6 : 165 - 165
  • [2] Identification of differentially expressed genes in the visual structures of brain using high-density cDNA grids
    Prasad, SS
    Kojic, LZ
    Lee, SS
    Chaudhuri, A
    Hetherington, P
    Cynader, MS
    MOLECULAR BRAIN RESEARCH, 2000, 82 (1-2): : 11 - 24
  • [3] Identification of differentially expressed genes in high-density oligonucleotide arrays accounting for the quantification limits of the technology
    Tadesse, MG
    Ibrahim, JG
    Mutter, GL
    BIOMETRICS, 2003, 59 (03) : 542 - 554
  • [4] Association of differentially expressed genes with activation of mouse hepatic stellate cells by high-density cDNA mircoarray
    Liu, Xiao-Jing
    Yang, Li
    Luo, Feng-Ming
    Wu, Hong-Bin
    Qu-Qiang
    WORLD JOURNAL OF GASTROENTEROLOGY, 2004, 10 (11) : 1600 - 1607
  • [5] Association of differentially expressed genes with activation of mouse hepatic stellate cells by high-density cDNA mircoarray
    Xiao-Jing Liu Hong-Bin Wu Qu Qiang Laboratory of Department of Internal Medicine
    World Journal of Gastroenterology, 2004, (11) : 1600 - 1607
  • [6] Analysis of differentially expressed genes in hepatocellular carcinoma using cDNA Arrays
    Goldenberg, D
    Ayesh, S
    Schneider, T
    Pappo, O
    Jurim, O
    Eid, A
    Fellig, Y
    Dadon, T
    Ariel, I
    de Groot, N
    Hochberg, A
    Galun, E
    MOLECULAR CARCINOGENESIS, 2002, 33 (02) : 113 - 124
  • [7] Rapid identification of allergen-encoding cDNA clones by phage display and high-density arrays
    Kodzius, R
    Rhyner, C
    Konthur, Z
    Buczek, D
    Lehrach, H
    Walter, G
    Crameri, R
    COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING, 2003, 6 (02) : 147 - 154
  • [8] Analysis of genes differentially expressed in astrocytes stimulated with lipopolysaccharide using cDNA arrays
    Pang, Y
    Cai, ZW
    Rhodes, PG
    BRAIN RESEARCH, 2001, 914 (1-2) : 15 - 22
  • [9] Identification of differentially expressed genes in gastric cancer by high density cDNA microarray
    Zhang, Song-Nan
    Sun, Hong-Hua
    Jin, Yong-Min
    Piao, Long-Zhen
    Jin, De-Hao
    Lin, Zhen-Hua
    Shen, Xiong-Hu
    CANCER GENETICS, 2012, 205 (04) : 147 - 155
  • [10] HIGH-DENSITY CDNA FILTER ANALYSIS OF THE EXPRESSION PROFILES OF THE GENES PREFERENTIALLY EXPRESSED IN HUMAN BRAIN
    TAKAHASHI, N
    HASHIDA, H
    ZHAO, ND
    MISUMI, Y
    SAKAKI, Y
    GENE, 1995, 164 (02) : 219 - 227
12345