Tumor necrosis factor-α enhances both epithelial-mesenchymal transition and cell contraction induced in A549 human alveolar epithelial cells by transforming growth factor-β1

被引：67
|
作者：
Yamauchi, Yasuhiro [1 ]
Kohyama, Tadashi [1 ]
Takizawa, Hajime [2 ]
Kamitani, Sumiko [1 ]
Desaki, Masashi [1 ]
Takami, Kazutaka [1 ]
Kawasaki, Shin [1 ]
Kato, Jun [1 ]
Nagase, Takahide [1 ]
机构：
[1] Univ Tokyo, Dept Resp Med, Tokyo, Japan
[2] Teikyo Univ, Dept Internal Med 4, Tokyo 173, Japan
来源：
EXPERIMENTAL LUNG RESEARCH | 2010年 / 36卷 / 01期
关键词：
alveolar epithelial cell; cell contractility; epithelial mesenchymal transition; pulmonary fibrosis; TGF-beta; 1; TNF-alpha; GROWTH-FACTOR-BETA; FIBROBLAST-MEDIATED CONTRACTION; IDIOPATHIC PULMONARY-FIBROSIS; TRANSCRIPTION FACTOR SNAIL; INTERFERON-GAMMA; GENE-EXPRESSION; GEL CONTRACTION; COLLAGEN GELS; TGF-BETA-1; GLUCOCORTICOIDS;
D O I：
10.3109/01902140903042589
中图分类号：
R56 [呼吸系及胸部疾病];
学科分类号：
摘要：
Recently, epithelial-mesenchymal transition (EMT) has been reported to contribute to tissue fibrosis through enhanced transforming growth factor (TGF)-beta 1 signaling. Tumor necrosis factor (TNF)-alpha has also been implicated in tissue fibrosis. Therefore, the authors investigated whether TNF-alpha affected TGF-beta 1-induced EMT. Cultured alveolar epithelial cells (A549 cells) were stimulated with TGF-beta 1 (5 ng/mL), with/without TNF-alpha (10 ng/mL). TGF-beta 1 induced EMT of A549 cells, with loss of E-cadherin and acquisition of vimentin. Combination of TNF-alpha with TGF-beta 1 enhanced EMT, causing morphological changes, while quantitative polymerase chain reaction (PCR) showed suppression of E-cadherin mRNA and expression of vimentin mRNA. In addition, the gel contraction method revealed that cells that had undergone EMT acquired cell contractility, which is a feature of mesenchymal cells. Stimulation with TGF-beta 1 induced cell contraction, as did TNF-alpha. Moreover, costimulation with TGF-beta 1 and TNF-alpha enhanced the cell contraction. Although IFN-gamma suppressed spontaneous cell contraction, it did not suppress cell contraction, which was induced by TGF-beta 1. In conclusion, TNF-alpha enhances not only EMT but also cell contraction induced by TGF-beta 1. EMT might contribute to tissue fibrosis through induction of cell contraction.
引用
收藏
页码：12 / 24
页数：13
相关论文
共 50 条
  • [1] TUMOR NECROSIS FACTOR SUPER FAMILY MEMBER LIGHT ENHANCES EPITHELIAL-MESENCHYMAL TRANSITION INDUCED IN A549 HUMAN ALVEOLAR EPITHELIAL CELLS BY TRANSFORMING GROWTH FACTOR-BETA 1
    Mikami, Y.
    Yamauchi, Y.
    Kohyama, T.
    Horie, M.
    Saito, A.
    Jo, T.
    Takizawa, H.
    Nagase, T.
    [J]. RESPIROLOGY, 2011, 16 : 23 - 23
  • [2] Epithelial-mesenchymal transition induced by transforming growth factor-β1 in mouse tracheal epithelial cells
    Kuroishi, Shigeki
    Suda, Takafumi
    Fujisawa, Tomoyuki
    Ide, Kyotaro
    Inui, Naoki
    Nakamura, Yutaro
    Nakamura, Hirotoshi
    Chida, Kingo
    [J]. RESPIROLOGY, 2009, 14 (06) : 828 - 837
  • [3] Tumor necrosis factor superfamily member LIGHT induces epithelial-mesenchymal transition in A549 human alveolar epithelial cells
    Mikami, Yu
    Yamauchi, Yasuhiro
    Horie, Masafumi
    Kase, Makiko
    Jo, Taisuke
    Takizawa, Hajime
    Kohyama, Tadashi
    Nagase, Takahide
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2012, 428 (04) : 451 - 457
  • [4] Role of β-Catenin Signaling in Transforming Growth Factor-β1-Induced Epithelial-Mesenchymal Transition of Alveolar Epithelial Cells.
    Zhou, B.
    Han, A.
    Zhong, Q.
    Ann, D. K.
    Minoo, P.
    Liebler, J. M.
    Crandall, E. D.
    Kahn, M.
    Borok, Z.
    [J]. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2009, 179
  • [5] Transforming Growth Factor-β1 Induced Epithelial-Mesenchymal Transition in Hepatic Fibrosis
    Bi, Wan-Rong
    Yang, Chang-Qing
    Shi, Qing
    [J]. HEPATO-GASTROENTEROLOGY, 2012, 59 (118) : 1960 - 1963
  • [6] Overexpression of heat shock protein 70 inhibits epithelial-mesenchymal transition and cell migration induced by transforming growth factor-β in A549 cells
    Shi, Fengxian
    Ma, Mingze
    Zhai, Ruonan
    Ren, Yanan
    Li, Ke
    Wang, Hang
    Xu, Chunyan
    Huang, Xiaowen
    Wang, Na
    Zhou, Fang
    Yao, Wu
    [J]. CELL STRESS & CHAPERONES, 2021, 26 (03): : 505 - 513
  • [7] Overexpression of heat shock protein 70 inhibits epithelial-mesenchymal transition and cell migration induced by transforming growth factor-β in A549 cells
    Fengxian Shi
    Mingze Ma
    Ruonan Zhai
    Yanan Ren
    Ke Li
    Hang Wang
    Chunyan Xu
    Xiaowen Huang
    Na Wang
    Fang Zhou
    Wu Yao
    [J]. Cell Stress and Chaperones, 2021, 26 : 505 - 513
  • [8] The Cain and Abl of Epithelial-Mesenchymal Transition and Transforming Growth Factor-β in Mammary Epithelial Cells
    Allington, Tressa M.
    Schiemann, William P.
    [J]. CELLS TISSUES ORGANS, 2011, 193 (1-2) : 98 - 113
  • [9] Interactions of Retinoic Acid and Transforming Growth Factor-β1 on the effect of Epithelial-Mesenchymal Transition in Human Epithelial Cell
    Lee, S. K.
    Lee, S. H.
    Kim, S. Y.
    Lim, J. E.
    Jung, J. Y.
    Park, B. H.
    Kang, Y. A.
    Kim, S. K.
    Chang, J.
    Park, M. S.
    [J]. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2010, 181
  • [10] Hepatocyte Growth Factor (HGF) Attenuates Transforming Growth Factor-β-Induced Epithelial Mesenchymal Transition in Alveolar Epithelial Cells.
    Krishnaveni, M. S.
    Zhou, B.
    Willis, B. C.
    Crandall, E. D.
    Borok, Z.
    [J]. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2009, 179
12345