GENETIC VARIATION IN CYPRINION MACROSTOMUS HECKEL, 1843 POPULATIONS AS REVEALED BY PARTIAL COI SEQUENCES OF MITOCHONDRIAL DNA

Cyprinion macrostomus Heckel, 1843 naturally thriving in river systems of Euphrates and Tigris is a species with economic importance. In this study genetic diversity of C. macrostomus populations was determined based on gene sequencing analysis of mitochondrial DNA cytochrome c oxidase subunit I (mtDNA COI) locus. Seven polymorphic sites and eight haplotypes were detected taking 41 samples from two populations. Mean haplotype diversity (h) and nucleotide diversity (pi) were calculated to be h = 0.529 and pi = 0.00158; respectively. All values obtained from two populations after neutrality tests were calculated to be negative and were statistically insignificant (p > 0.05). Results obtained with this research are the data noted for the first time for C. macrostomus species thriving in Turkey. Certain haplotypes (H3, H5, H6, H7 and H8) determined for mtDNA COI locus are the new results to the literature and created a novel data set for genetic diversity of this species.