Occurrence and die-off of indicator organisms in the sediment in two constructed wetlands

The interest in constructed wetlands for municipal wastewater and stormwater treatment has recently increased but data for the reduction efficiency of indicator organisms are often restricted to the water phase. In a full-scale wastewater wetland in Sweden fecal coliforms and enterococci were reduced by 97-99.9% and coliphages by approximately 70%. The factors affecting the reduction are however less well understood. In two full-scale wetlands, for stormwater and wastewater treatment, an assessment has been done of the particle associated fraction of indicator organisms. No significant differences in the particle-associated numbers were seen between the inlet and the outlet of the wetlands, but the amounts of sedimenting particles varied between the two sites. In the stormwater wetland the amount of sedimenting particles at the outlet was 3% of the amount at the inlet, while the wastewater wetland had much lower particle removal efficiency. The reduction of suspended particles seems to be the main factor for bacterial elimination from the water phase, governed by vegetation and design. In the sediment, survival of presumptive E. coli, fecal enterococci, Clostridium and coliphages were long with T-90-values of 27, 27, 252 and 370 days, respectively. The organisms can however be reintroduced by resuspension. Viruses in the water phase may be of main concern for a risk assessment of receiving waters.