AFLP analysis of genetic diversity within a jackfruit germplasm collection

Twenty-six jackfruit accessions, one interspecific hybrid, champedak, and one breadfruit accession were analyzed using amplified fragment length polymorphic (AFLP) markers to determine the degree of genetic diversity within the Fairchild Tropical Garden (FTG) germplasm. collection. Of the 30 primer pairs evaluated, 12 were identified for collection screening based on Dumber and quality of polymorphic fragments. A total of 187 AFLP markers were scored using the 12 primer pairs, 92 (49.2%) being polymorphic. All accessions could be uniquely identified using the 12 primer pairs. Among the jackfruit accessions, similarity coefficients ranged from 0.567 to 0.950; the accessions also shared a large number of monomorphic fragments (54.9%). Cluster analysis and principal component analysis (PCA) grouped all of the jackfruit accessions with southeast Asian origins into one major cluster with little bootstrap support for groupings within the cluster. The Indian accessions were grouped in a different cluster, as did the hybrid and the breadfruit accession. The AFLP marker based analysis indicates that limited genetic diversity exists within this collection. These observations are in agreement with the phenotypic evaluation and suggest that new accessions be obtained from the center of origin for the species. Published by Elsevier Science B.V.