Heparan sulfate C5-epimerase is essential for heparin biosynthesis in mast cells

被引：43
|
作者：
Feyerabend, TB
Li, JP
Lindahl, U
Rodewald, HR
机构：
[1] Univ Uppsala, Dept Med Biochem & Microbiol, Biomed Ctr, SE-75123 Uppsala, Sweden
[2] Univ Ulm, Dept Immunol, D-89070 Ulm, Germany
来源：
NATURE CHEMICAL BIOLOGY | 2006年 / 2卷 / 04期
关键词：
D O I：
10.1038/nchembio777
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Biosynthesis of heparin, a mast cell-derived glycosaminoglycan with widespread importance in medicine, has not been fully elucidated. In biosynthesis of heparan sulfate (HS), a structurally related polysaccharide, HS glucuronyl C5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) residues. We have generated Hsepi-null mouse mutant mast cells, and we show that the same enzyme catalyzes the generation of IdoA in heparin and that 'heparin' lacking IdoA shows a distorted O-sulfation pattern.
引用
收藏
页码：195 / 196
页数：2
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