HPTLC-Densitometry Method for Simultaneous Determination of Major Lignans and Flavonoids in Podophyllum hexandrum

被引:1
|
作者
Chaudhary, Abha [1 ]
Kaur, Pushpinder [1 ]
Katiyar, Arti [1 ]
Singh, Bikram [1 ]
机构
[1] CSIR Inst Himalayan Bioresource Technol, Nat Plant Prod Div, Palampur 176061, Himachal Prades, India
关键词
HPTLC; Lignans; Flavonoid; Podophyllum hexandrum; RAPID-DETERMINATION; HPLC;
D O I
10.1556/JPC.25.2012.4.6
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new quantitative method using thin-layer chromatography silica gel 60F(254) plates as the stationary phase and the mobile phase consisting of toluene-ethyl acetate-acetic acid (15.0:7.5:0.5, v/v) was employed for simultaneous determination of lignans and flavonoid in Podophyllum hexandrum. Densitometric determination of the constituents was performed at 254 nm in reflectance/absorbance mode. The method was validated for precision, accuracy, specificity, robustness, and recovery. The linear regression analysis data for the calibration plots showed a good linear relationship (r(2) = 0.9903-0.9976) in the calibration range of 1-8 mu g per band for 4'-O-demethylpodophyllotoxin (1), podophyllotoxin (2), and podophyllotoxone (4), and 2-10 mu g per band for kaempferol (3) and deoxypodophyllotoxin (5) with respect to peak area. Limits of detection and quantitation were in the range of 250-617 ng per band and 856-1974 ng per band. The average recovery for 4'-O-demethylpodophyllotoxin, podophyllotoxin, kaempferol, podophyllotoxone, and deoxypodophyllotoxin was 96.38 +/- 1.92 to 101.84 (+/-) 1.05%, indicating the excellent reproducibility. Podophyllotoxin was found in highest content (9.92 mu g mg(-1)) and podophyllotoxone in the lowest content (0.94 mu g mg(-1)). The proposed method is rapid, simple, precise, specific, sensitive, accurate, and robust.
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页码:314 / 319
页数:6
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