Molecular characterization of G2P[4] and G9P[8] rotaviruses strains isolated from Moroccan children fully vaccinated with Rotarix™ between 2013 and 2014

被引：0

作者：

Boulahyaoui, Hassan ^{[1
,2
]}

Amine, Sanaa Alaoui ^{[3
,4
]}

Melloul, Marouane ^{[5
]}

Loutfi, Chafiqa ^{[6
]}

Tagajdid, Reda ^{[1
,2
]}

El Annaz, Hicham ^{[1
,2
]}

Touil, Nadia ^{[1
,7
]}

Malik, Yashpal Singh ^{[8
]}

El Fahime, Elmostafa ^{[1
,3
,4
]}

Mrani, Saad ^{[1
,2
]}

机构：

[1] Univ Mohamed, Fac Med & Pharm, Ctr Genom Pathol Humaines GENOPATH, Rabat, Morocco

[2] Hop Mil Instruct Mohamed V, Ctr Virol Malad Infect & Trop, Rabat, Morocco

[3] Ctr Natl Rech Sci & Tech, UATRS, Rabat, Morocco

[4] Univ Mohammed 5, Fac Med & Pharm, Lab Biotechnol Med, Rabat, Morocco

[5] Univ Mohammed Premier, Fac Sci, Lab Physiol Genet & Ethnopharmacol, Oujda, Morocco

[6] Soc Prod Biol & Pharmaceut Vet, Dept Virol, Rabat, Morocco

[7] Hop Mil Instruct Med V, Lab Rech & Biosecurite, Rabat, Morocco

[8] Indian Vet Res Inst, Izatnagar, Uttar Pradesh, India

来源：

ALEXANDRIA JOURNAL OF MEDICINE | 2019年 / 55卷 / 01期

关键词：

Rotarix; G2P[4] and G9P[8] genotypes; phylogenetic analysis; VP8* neutralizing domains; Morocco; ACUTE GASTROENTERITIS; SEVERE DIARRHEA; AGE; DIVERSITY;

D O I：

10.1080/20905068.2019.1592887

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: Here, we describe the characterization of the VP7 and VP4 genes of rotavirus group A (RVA) strains isolated from Moroccan infants with acute gastroenteritis (AGE) after receiving the Rotarix. Methods: Stool samples were collected from seven infants suffering with AGE. Vesikari 20-point was used while collecting the AGE information. RT-PCR followed by sequencing was employed to determine partial sequences from VP7 and VP4 encoding genes. RVA genotypes were determined using the RotaC online classification tool.The Neighbor-Joining phylogenetic tree was performed in the MEGA 6.06 software as well as the amino acid (aa) alignments of VP7 and VP8* antigenic epitopes. Results: Five children had gastroenteritis episodes defined as severe on the Vesikari scale. Analysis of VP7 gene sequences revealed five G2 and two G9 genotypes. These genotypes were associated with P[4] and P[8], respectively. Phylogenetic analysis demonstrated that G2 and G9 sequences grouped together with Italian strain belonging to lineage II and III respectively. Their P[4] sequences showed only 65-73% identity and grouped into two separate sub-clusters. The Moroccan P[8] isolates did not display this correlation and segregate to lineage III. The VP8* aa of P[8] when compared with that of the Rotarix vaccine, has a six aa differences. Conclusion: Although, this study does not reveal prevalence rates of RVA in infants after the vaccine introduction, it reports the detection and genetic characteristics of the G2P[4] and G9P[8] RVA types in vaccinated children in Morocco. The findings are suggestive of continuous surveillance program on rotavirus evolution in the vaccinated population.

引用

页码：7 / 14

页数：8

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