Linkage map of Syrian hamster with restriction landmark genomic scanning

被引：11

作者：

Okuizumi, H

Ohsumi, T

Sasaki, N

Imoto, H

Mizuno, Y

Hanami, T

Yamashita, H

Kamiya, M

Takada, S

Kitamura, A

Muramatsu, M

Nishimura, M

Mori, M

Matsuda, Y

Tagaya, O

Okazaki, Y

Hayashizaki, Y

机构：

[1] RIKEN, GENOME SCI LAB, TSUKUBA LIFE SCI CTR, TSUKUBA, IBARAKI 305, JAPAN

[2] FORESTRY & FOREST PROD RES INST, ECOL GENET LAB, TSUKUBA, IBARAKI 305, JAPAN

[3] HAMAMATSU UNIV SCH MED, INST EXPT ANIM, HAMAMATSU, SHIZUOKA 43131, JAPAN

[4] NAGOYA UNIV, SCH AGR SCI, ANIM GENE CONTROL LAB, NAGOYA, AICHI 46401, JAPAN

[5] EISAI & CO LTD, DEPT PLANNING & RESOURCES, DIV RES, BUNKYO KU, TOKYO 11288, JAPAN

来源：

MAMMALIAN GENOME | 1997年 / 8卷 / 02期

关键词：

D O I：

10.1007/s003359900370

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have constructed the linkage map with precise genetic analysis of the Syrian hamster, Mesocricetus auratus, according to the restriction landmark genomic scanning (RLGS) spot mapping method. Although only 3.2-6.6% of the total RLGS spots between the two strains, ACN and BIO 14.6, showed genetic variance, 572 loci were found to be polymorphic. Out of 569 RLGS loci and 3 other loci, 531 were mapped with the backcross (ACN x BIO 14.6) F-1 x BIO 14.6. The cumulative map was 1111.6 cM, indicating that the spots/loci are located throughout the genome at 1.94 cM intervals on average. Thus, RLGS provides us with a rapid tool to construct the genetic map of any species, even if it has less genetic variation.

引用

页码：121 / 128

页数：8

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