Frequency of antibodies against Besnoitia besnoiti in Brazilian cattle

被引：10
|
作者：
Uzeda, R. S. [1 ]
Andrade, M. R. [1 ]
Corbellini, L. G. [2 ]
Antonello, A. M. [3 ]
Vogel, F. S. [3 ]
Gondim, L. F. P. [1 ]
机构：
[1] Univ Fed Bahia, Escola Med Vet & Zootecnia, BR-40170110 Salvador, BA, Brazil
[2] Univ Fed Rio Grande do Sul, Dept Med Vet Prevent, Fac Med Vet, BR-91540000 Porto Alegre, RS, Brazil
[3] Univ Fed Santa Maria, Ctr Ciencias Rurais, Dept Med Vet Prevent, BR-97105900 Santa Maria, RS, Brazil
来源：
VETERINARY PARASITOLOGY | 2014年 / 199卷 / 3-4期
关键词：
Besnoitia besnoiti; Cattle; IFAT; Western blot; Brazil; BOVINE BESNOITIOSIS; NEOSPORA-CANINUM; INFECTED CATTLE; GERMANY;
D O I：
10.1016/j.vetpar.2013.10.025
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Besnoitia besnoiti is a cyst-forming parasite that has been associated with economic losses in Africa and Europe. Besnoitiosis is considered as a re-emergent disease in the European continent. It is unknown whether cattle are exposed to B. besnoiti in the Americas, thus the aim of this study was to serologically investigate antibodies against B. besnoiti in a total of 2014 cattle serum samples from two states from Brazil. All samples were evaluated by IFAT and part of the positive sera was tested by Western blot (WB) using tachyzoites extracts under non-reducing condition. A total of 3.48% (70/2014) of the tested sera reacted positively by IFAT with titers of 200 (85.7%), 400 (10%) and 800 (4.3%). When 47 positive samples were assessed by WB a range of antigens from 7 to 206 kDa was recognized by the IFAT-positive sera. The results are suggestive of exposure of Brazilian cattle to B. besnoiti due to the titers (>= 200) observed for some sera using IFAT. However, the antigens recognized by the IFAT-positive animals did not completely match with the WB patterns previously described by other working groups. It is possible that Brazilian cattle are exposed to B. besnoiti strains with different antigenic composition of-those described in the European and African continent. Further studies are needed to confirm the presence of B. besnoiti or other Besnoitia species in Brazilian cattle. (C) 2013 Elsevier B.V. All rights reserved.
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页码：242 / 246
页数：5
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