Nucleobase mapping of self-assembled monolayers by chemical force microscopy

被引:7
|
作者
Sunami, H [1 ]
Ijiro, K
Karthaus, O
Kraemer, S
Mittler, S
Knoll, W
Simomura, M
机构
[1] Hokkaido Univ, Res Inst Elect Sci, Sapporo, Hokkaido 0600812, Japan
[2] Max Planck Inst Polymer Res, D-55128 Mainz, Germany
关键词
chemical force microscopy; molecular recognition; force mapping; self-assembled monolayers; hydrogen bond; DNA;
D O I
10.1080/10587250108024710
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In order to measure complementary hydrogen bonding between nucleobases, disulfide derivatives of thymine and adenine were newly synthesized. Surface Plasmon Resonance; SPR confirmed the formation of SAMs on Au. SPR shows that SAMs of nucleobase derivatives were completely formed within 60 minutes. Thickness of the adenine SAM and the thymine SAM is nearly the same. AFM tip was modified with the thymine SAM. The adenine SAM-modified Au electrode was clearly observed in an adhesion force map. The adhesion force between complementary nucleobases is larger than that of the non-complementary combination.
引用
收藏
页码:151 / 154
页数:4
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