Biochemical evaluation of the effects of storage on feline erythrocytes

OBJECTIVE : To describe the biochemical changes that occur during storage of feline packed red blood cells. METHODS : Feline packed red blood cells were obtained from the manufacturer via overnight delivery immediately following collection. Bag spikes were placed using aseptic technique and samples were drawn on days 1, 4, 7, 14, 21, 28 and 35. Sodium, potassium, chloride, glucose, lactate, pH and ammonia were measured at each time point. Aerobic and anaerobic bacterial cultures were submitted following collection on day 35. RESULTS : There were statistically significant increases in the median concentrations of lactate and ammonia within the first 2 weeks of storage to a concentration of 12.38 mmol/L and 447.96 mu mol/L, respectively. Glucose concentrations decreased significantly by day 28 to a mean of 1.86 mmol/L. Median sodium and chloride concentrations increased throughout the course of storage to a concentration of 158.20 and 131.00 mmol/L, respectively. Mean potassium concentrations decreased to a concentration of 2.40 mmol/L. CLINICAL SIGNIFICANCE : These results show that biochemical derangements within feline packed red blood cells are progressive, with some alterations, such as lactate and ammonia, occurring early within the storage periods, while others, including glucose and electrolytes, are slower to develop. Additional prospective research evaluating the clinical effects of these biochemical alterations is required.