lncRNA TTN-AS1 upregulates RUNX1 to enhance glioma progression via sponging miR-27b-3p

被引:22
|
作者
Chang, Keliang [1 ]
Wang, Genwei [1 ]
Lou, Jinfeng [1 ]
Hao, Sha [2 ]
Lv, Ranbo [3 ]
Duan, Desheng [4 ]
Zhang, Wanhong [5 ]
Guo, Yingchang [6 ]
Wang, Pengfei [7 ]
机构
[1] Zhengzhou Univ, Dept Neurosurg, Affiliated Hosp 2, Zhengzhou 450014, Henan, Peoples R China
[2] Tradit Chinese Med Hosp Jingmen, Dept Oncol, Jingmen 448000, Hubei, Peoples R China
[3] Longhai Hosp Kaifeng City, Dept Neurosurg, Kaifeng 475000, Henan, Peoples R China
[4] Third Peoples Hosp Anyang, Dept Orthopaed, Anyang 455000, Henan, Peoples R China
[5] Kaifeng Cent Hosp, Dept Neurosurg, Kaifeng 475000, Henan, Peoples R China
[6] Xinxiang Med Univ, Dept Intervent Therapy, Affiliated Hosp 1, 88 Jiankang Rd, Weihui 453100, Henan, Peoples R China
[7] Peoples Hosp Pingyu, Dept Neurosurg, 93 Jiankang Rd, Pingyu 463400, Henan, Peoples R China
关键词
glioma; TTN-AS1; miR-27b-3p; RUNX1; LONG NONCODING RNA; CELL-PROLIFERATION; EXPRESSION; CARCINOMA; INVASION; CANCER; TRANSCRIPTION; MIGRATION; INTERACTS; GROWTH;
D O I
10.3892/or.2020.7684
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Long non-coding RNAs (lncRNAs) contribute to the tumorigeneses of numerous types of cancer, including glioma. The present study was designed to unveil a novel lncRNA functioning in glioma and explore the underlying mechanisms. lncRNA titin-antisense RNA1 (TTN-AS1), miR-27b-3p and Runt-related transcription factor 1 (RUNX1) expression in glioma tissues and cell lines was estimated by RT-qPCR. Si-TTN-AS1 was transfected into glioma cell lines (U251 and LN229), and CCK-8 assay, flow cytometry, wound healing and Transwell assays were applied to estimate the function of TTN-AS1 in glioma cells. miR-27b-3p inhibitor was used to explore the mechanisms. The results revealed that TTN-AS1 was highly expressed in glioma specimens and cell lines. Downregulation of TTN-AS1 inhibited the proliferation, migration and invasion of the glioma cells, as well as increased the rate of apoptosis.In vivo, the tumor growth was also inhibited by TTN-AS1 depletion in nude mice. Furthermore, we revealed that TTN-AS1 exerted oncogenic effects via sponging miR-27b-3p and thereby positively regulating RUNX1 expression. In conclusion, the present study supported that TTN-AS1 acts as an oncogene in glioma by targeting miR-27b-3p to release RUNX1. This finding may contribute to gene therapy of glioma.
引用
收藏
页码:1064 / 1074
页数:11
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