Clonal analysis of non-toxigenic Vibrio cholerae O1 associated with an outbreak of cholera

被引:0
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作者
Pal, A
Saha, PK
Nair, GB
Yamasaki, S
Takeda, T
Takeda, Y
Bhattacharya, SK
Ramamurthy, T
机构
[1] Natl Inst Cholera & Enter Dis, Calcutta, W Bengal, India
[2] Natl Childrens Med Res Ctr, Dept Infect Dis Res, Tokyo 154, Japan
[3] Int Med Ctr Japan, Res Inst, Tokyo, Japan
关键词
non-toxigenic (NT) Vibrio cholerae O1; phylogenetic analysis; pulsed-field gel electrophoresis (PFGE);
D O I
暂无
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We examined the clonal relationships among eight clinical isolates of non-toxigenic (NT) V. cholerae O1 associated with a cluster of cases of cholera in Warangal, Andhra Pradesh in south India and compared their relatedness to toxigenic O1 strains of classical and ElTor biotypes and with O139 Bengal strains of II cholerae by pulsed-field gel electrophoresis (PFGE). Phylogentic analysis of the NotI re-striction fragment length polymorphism showed that all the NT. V. cholerae O1 strains formed a tight cluster with more than 80 per cent similarity. Interestingly, the NT V. cholerae O1 cluster was more closely related to V: cholerae O139 than to classical and ElTor biotypes of V. cholerae O1 indicating closer genetic relationships between NT I! cholerae 01 and O139 Bengal strains that were Isolated during the same time-frame.
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页码:208 / 211
页数:4
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