Mercuric Compounds Induce Pancreatic Islets Dysfunction and Apoptosis in Vivo

被引:16
|
作者
Chen, Kuo-Liang [2 ,3 ]
Liu, Shing-Hwa [4 ]
Su, Chin-Chuan [5 ]
Yen, Cheng-Chieh [6 ,7 ]
Yang, Ching-Yao [8 ,9 ]
Lee, Kuan-I [10 ]
Tang, Feng-Cheng [11 ]
Chen, Ya-Wen [12 ,13 ]
Lu, Tien-Hui [12 ,13 ]
Su, Yi-Chang [1 ]
Huang, Chun-Fa [1 ]
机构
[1] China Med Univ, Coll Chinese Med, Sch Chinese Med, Taichung 404, Taiwan
[2] China Med Univ, China Med Univ Hosp, Dept Urol, Taichung 404, Taiwan
[3] China Med Univ, Sch Med, Taichung 404, Taiwan
[4] Natl Taiwan Univ, Coll Med, Inst Toxicol, Taipei 100, Taiwan
[5] Changhua Christian Hosp, Dept Otorhinolaryngol Head & Neck Surg, Changhua 500, Taiwan
[6] Chung Shan Med Univ, Coll Hlth Care & Management, Dept Occupat Safety & Hlth, Taichung 402, Taiwan
[7] Chung Shan Med Univ Hosp, Dept Occupat Med, Taichung 402, Taiwan
[8] Natl Taiwan Univ, Natl Taiwan Univ Hosp, Dept Surg, Taipei 10043, Taiwan
[9] Natl Taiwan Univ, Dept Surg, Coll Med, Taipei 10043, Taiwan
[10] Buddhist Tzu Chi Gen Hosp, Taichung Branch, Dept Emergency, Taichung 427, Taiwan
[11] Changhua Christian Hosp, Dept Occupat Med, Changhua 500, Taiwan
[12] China Med Univ, Dept Physiol, Taichung 404, Taiwan
[13] China Med Univ, Grad Inst Basic Med Sci, Sch Med, Coll Med, Taichung 404, Taiwan
来源
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2012年 / 13卷 / 10期
关键词
mercuric compounds; pancreatic islets; oxidative stress; apoptosis; INDUCED OXIDATIVE STRESS; GLUCOSE-TRANSPORT; 3T3-L1; ADIPOCYTES; CELL APOPTOSIS; METHYLMERCURY; MICE; ACTIVATION; EXPRESSION; METALS; P38;
D O I
10.3390/ijms131012349
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mercury is a toxic heavy metal that is an environmental and industrial pollutant throughout the world. Mercury exposure leads to many physiopathological injuries in mammals. However, the precise toxicological effects of mercury on pancreatic islets in vivo are still unclear. Here, we investigated whether mercuric compounds can induce dysfunction and damage in the pancreatic islets of mice, as well as the possible mechanisms involved in this process. Mice were treated with methyl mercuric chloride (MeHgCl, 2 mg/kg) and mercuric chloride (HgCl2, 5 mg/kg) for more than 2 consecutive weeks. Our results showed that the blood glucose levels increased and plasma insulin secretions decreased in the mice as a consequence of their exposure. A significant number of TUNEL-positive cells were revealed in the islets of mice that were treated with mercury for 2 consecutive weeks, which was accompanied by changes in the expression of the mRNA of anti-apoptotic (Bcl-2, Mcl-1, and Mdm-2) and apoptotic (p53, caspase-3, and caspase-7) genes. Moreover, plasma malondialdehyde (MDA) levels increased significantly in the mice after treatment with mercuric compounds for 2 consecutive weeks, and the generation of reactive oxygen species (ROS) in the pancreatic islets also markedly increased. In addition, the mRNA expression of genes related to antioxidation, including Nrf2, GPx, and NQO1, were also significantly reduced in these islets. These results indicate that oxidative stress injuries that are induced by mercuric compounds can cause pancreatic islets dysfunction and apoptosis in vivo.
引用
收藏
页码:12349 / 12366
页数:18
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