Neutron diffraction analysis of Pseudomonas aeruginosa peptidyl-tRNA hydrolase 1

被引：3

作者：

McFeeters, Hana ^{[1
]}

Vandavasi, Venu Gopal ^{[2
]}

Weiss, Kevin L. ^{[2
]}

Coates, Leighton ^{[2
]}

McFeeters, Robert L. ^{[1
]}

机构：

[1] Univ Alabama, Dept Chem, 301 Sparkman Dr, Huntsville, AL 35899 USA

[2] Oak Ridge Natl Lab, Biol & Soft Matter Div, 1 Bethel Valley Rd, Oak Ridge, TN 37831 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2016年 / 72卷

关键词：

peptidyl-tRNA hydrolase 1; neutron diffraction; perdeuteration; Pseudomonas aeruginosa; antibiotic resistance; ESCHERICHIA-COLI; MINIGENE EXPRESSION; X-RAY; INHIBITION; CRYSTALLIZATION; PNEUMONIA; GROWTH;

D O I：

10.1107/S2053230X16001813

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Perdeuterated peptidyl-tRNA hydrolase 1 from Pseudomonas aeruginosa was crystallized for structural analysis using neutron diffraction. Crystals of perdeuterated protein were grown to 0.15 mm(3) in size using batch crystallization in 22.5% polyethylene glycol 4000, 100 mM Tris pH 7.5, 10% (v/v) isopropyl alcohol with a 20-molar excess of trilysine as an additive. Neutron diffraction data were collected from a crystal at room temperature using the MaNDi single-crystal diffractometer at Oak Ridge National Laboratory.

引用

页码：220 / 223

页数：4

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