Synthesis of Autophagosomal Marker Protein LC3-II under Detergent-Free Conditions

被引：97
|
作者：
Huang, Yi-Chao [1 ]
Li, Yi-Ming [2 ]
Chen, Yang [3 ]
Pan, Man [1 ]
Li, Yi-Tong [1 ]
Yu, Li [3 ]
Guo, Qing-Xiang [4 ]
Liu, Lei [1 ]
机构：
[1] Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Minist Educ,Dept Chem, Beijing 100084, Peoples R China
[2] Hefei Univ Technol, Sch Med Engn, Hefei 230009, Anhui, Peoples R China
[3] Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China
[4] Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 18期
关键词：
autophagy; LC3-II; lipoproteins; protein expression; protein ligation; TOTAL CHEMICAL-SYNTHESIS; SUPPORTED LIPID-BILAYERS; SIGNAL-TRANSDUCTION; ESCHERICHIA-COLI; IN-VITRO; LIGATION; BIOLOGY; SEMISYNTHESIS; MEMBRANES; PEPTIDES;
D O I：
10.1002/anie.201209523
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Just add oil: A new detergent-free method was developed to synthesize lipidated proteins using a light-activatable solubilizing side chain (in dashed circle, see scheme) to assist the ligation of the lipopeptides. This method allows the efficient preparation of a phosphatidylethanolamine-conjugated autophagosomal marker protein (LC3-II) as well as labeled derivatives of LC3-II, which can be used to study autophagy regulation. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：4858 / 4862
页数：5
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