Human albumin solders for clinical application during laser tissue welding

Background and Objective: Fifty percent human albumin solder significantly improves weld strength when compared to lower concentrations [Wright et al., ASLMS meeting, April, 1995]. We developed a method for preparing 50% human albumin that may be considered compatible for clinical applications. Study Design/Materials and Methods: Fifty percent human albumin solder was prepared from 25% commercially available human albumin using a lyophilization technique. Assessment of sterility, viscosity, pH, and peak absorption wavelength were performed. Results: This report describes the methology used to prepare a 50% human albumin solder that is compatible with clinical use, Maintenance of the structural integrity of the albumin was confirmed by polyacrylamide gel electrophoresis. Conclusion: This solder preparation can be used alone or with the addition of exogenous chromophores. The final product is sterile, incorporates viral free protocols, maintains high viscosity, and can be applied easily during open or laparoscopic procedures. (C) 1996 Wiley-Liss, Inc.