Effect of Temperature on Schizophyllum commune Growth and 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl-Production using a Bubble Column Bioreactor

Schizophyllum commune, a basidiomycetes species, exhibited the anti-fungal properties by producing several bioactive compounds during secondary metabolism. One flavonoid fraction, named 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl-(DDMP), has received much attention for its antifungal activity toward wood-degrading fungi of rubberwood. In the present study, the effect of temperature on the growth and bioactive compound production by Schizophyllum commune was investigated in a bubble column bioreactor. Results revealed that the highest production of biomass (35.11 +/- 0.12 g/L), total flavonoids (1.328 +/- 0.007 mu g QE/mg sample), and DDMP (1.278 +/- 0.005 mu g/mg sample) were achieved in the culture grown at 30 degrees C. The separation of crude extract was studied using a high performance liquid chromatography (HPLC) system equipped with a Zorbax XDB C18 column.