Transformation of the fungus Absidia glauca by complementation of a methionine-auxotrophic strain affected in the homoserine-acetyltransferase gene

被引：1

作者：

Karimi, Sedighe ^{[1
]}

Wetzel, Jana ^{[1
]}

Woestemeyer, Johannes ^{[1
]}

Burmester, Anke ^{[1
]}

机构：

[1] Univ Jena, D-07743 Jena, Germany

来源：

FEBS OPEN BIO | 2012年 / 2卷

关键词：

Homoserine O-acetyltransferase; Met2; Methionine biosynthesis; Mucor-like fungi; Zygomycetes;

D O I：

10.1016/j.fob.2012.07.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transformation of fungi by complementation of auxotrophs is generally much more reliable than usage of antibiotic resistance markers. In order to establish such a system for the model zygomycete Absidia glauca, a stable methionine auxotrophic mutant was isolated after X-ray mutagenesis of the minus mating type and characterized at the molecular level. The mutant is disrupted in the coding region of the Met2-1 gene, encoding homoserine O-acetyltransferase. The corresponding wild type gene was cloned, sequenced and inserted into appropriate vector plasmids. Transformants are prototrophs and show restored methionine-independent growth, based on complementation by the autonomously replicating plasmids. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：197 / 201

页数：5