High Sensitivity Detection of Gene-like Tumor Markers Based on SERS Characteristics of Hollow Sea-urchin Gold Nanoparticles and Noble Metal/semiconductor Substrate

被引：3

作者：

Peng Le ^{[1
]}

Zhou Lu ^{[2
]}

Qing Yan-ping ^{[3
]}

Tong Li-ying ^{[1
]}

Liang Zhao-heng ^{[1
]}

Fu Hao ^{[1
]}

Zhou Jun ^{[1
]}

机构：

[1] Ningbo Univ, Dept Microelect Engn, Sch Phys & Sci, Ningbo 315211, Zhejiang, Peoples R China

[2] Tianjin Univ, Ctr Terahertz Waves, Coll Precis Instrument & Optoelect Engn, Tianjin 300072, Peoples R China

[3] Ningbo Univ, Affiliated Hosp, Sch Med, Ningbo 315020, Zhejiang, Peoples R China

来源：

ACTA PHOTONICA SINICA | 2020年 / 49卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Surface-enhanced Raman scattering; Ag/ZnO nanostructure; Hollow sea-urchin gold nanoparticles; Gene-like tumor marker; MicroRNA; MICRORNAS; NANOGAP;

D O I：

10.3788/gzxb20204908.0817002

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Trace detection of genetic tumor marker microRNA (miRNA) has important application value for early diagnosis of cancer. According to the Surface-enhanced Raman Scattering characteristics of hollow sea urchin gold nanoparticles and Ag/ZnO nanostructures, and based on the principle of complementary base pairing, a "sandwich" structure of probe-nucleic acid-substrate is constructed and a highly sensitive quantitative detection scheme for genetic tumor marker miRNA is proposed. First, the captured DNA is linked to the hollow sea urchin gold nanoparticles modified with 4-mercaptobenzoic acid (4-MBA) as a probe. At the same time, the target DNA is modified on the Ag/ZnO nanostructure, and the SERS signal is detected to obtain the corresponding dose-response curve after complementary hybridization with miRNA-106a. The experimental results show that the detection limit of miRNA-106a reached 1.84 fmol.L-1 within the detection range of 1 fmol.L-1 similar to 1 nmol.L-1 . Meanwhile, the reliability of the miRNA detection scheme based on the hollow sea urchin gold nanoparticles and Ag/ZnO nano-structure SERS characteristics was verified by the Real-time quantitative Polynucleotide Chain Reaction (RT-qPCR) method.

引用

页数：10

共 29 条

[1] Probing microRNAs with microarrays: Tissue specificity and functional inference
Babak, T
Zhang, W
Morris, Q
Blencowe, BJ
Hughes, TR
[J]. RNA, 2004, 10 (11) : 1813 - 1819
[2] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004)
Bartel, David P.
[J]. CELL, 2007, 131 (04) : 11 - 29
[3] Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia
Calin, GA
Dumitru, CD
Shimizu, M
Bichi, R
Zupo, S
Noch, E
Aldler, H
Rattan, S
Keating, M
Rai, K
Rassenti, L
Kipps, T
Negrini, M
Bullrich, F
Croce, CM
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (24) : 15524 - 15529
[4] Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods
Ferlay, J.
Colombet, M.
Soerjomataram, I.
Mathers, C.
Parkin, D. M.
Pineros, M.
Znaor, A.
Bray, F.
[J]. INTERNATIONAL JOURNAL OF CANCER, 2019, 144 (08) : 1941 - 1953
[5] Highly Sensitive Detection of Tumor Marker miRNA-21 Based on Sandwich SERS Structure and Enzymatic Cleavage Technique
Fu Hao
Lu Wei-feng
Gao Yong-feng
Wang Zhe
Zou Zu-quan
Zhang Xin
Zhou Jun
[J]. ACTA PHOTONICA SINICA, 2019, 48 (07)
[6] [洑颢 Fu Hao], 2019, [发光学报, Chinese Journal of Luminescence], V40, P317
[7] Gupta V., 2011, Chron. Young Sci., V2, P21, DOI [10.4103/2229-5186.79345, DOI 10.4103/2229-5186.79345]
[8] Vertically Oriented Sub-10-nm Plasmonic Nanogap Arrays
Im, Hyungsoon
Bantz, Kyle C.
Lindquist, Nathan C.
Haynes, Christy L.
Oh, Sang-Hyun
[J]. NANO LETTERS, 2010, 10 (06) : 2231 - 2236
[9] Jemal A, 2011, CA-CANCER J CLIN, V61, P134, DOI [10.3322/caac.20115, 10.3322/caac.20107, 10.3322/caac.21492]
[10] A microRNA detection system based on padlock probes and rolling circle amplification
Jonstrup, Soren Peter
Koch, Jorn
Kjems, Jorgen
[J]. RNA, 2006, 12 (09) : 1747 - 1752

← 1 2 3 →