One-step self-assembled epoxide-containing nanodots as an enzyme-immobilized platform for biosensing

3-Glycidoxypropyl trimethoxysilane-containing self-assembled SiOx nanodots (GPS-SANDs) bearing epoxide functional groups were successfully fabricated via a facile one-step heat treatment process. The GPS-SANDs possessed spherical-like particles with an average diameter of 3.1 +/- 0.3 nm. This material exhibited a broad band, and a multicolor. Glucose oxidase (GOx) enzyme was further immobilized on GPS-SANDs via aminolysis of the epoxide groups to detect glucose using photoluminescence (PL) spectroscopy. The PL intensity changed inversely with the glucose concentration. The glucose detection limit of GOx-immobilized GPS-SANDS was determined to be 100 mu M. Therefore, GPS-SANDs provide a promising GOx-immobilized platform for the design of optical glucose biosensors with high sensitivity. In addition, this platform may allow for the further development of enzyme biosensing systems through conjugations between GPS-SANDS and a target enzyme. (C) 2016 Elsevier B.V. All rights reserved.