Effect of SNPs in protein kinase Cz gene on gene expression in the reporter gene detection system

被引：0
|
作者：
Liu, Zhuo
Sun, Hong-Xia
Zhang, Yong-Wei
Li, Yun-Feng
Zuo, Jin
Meng, Yan
Fang, Fu-De [1 ]
机构：
[1] Chinese Acad Med Sci, Natl Lab Med Mol Biol, Inst Basic Med Sci, Beijing 100005, Peoples R China
来源：
WORLD JOURNAL OF GASTROENTEROLOGY | 2004年 / 10卷 / 16期
基金：
国家高技术研究发展计划(863计划); 中国国家自然科学基金;
关键词：
D O I：
暂无
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
AIM: To investigated the effects of the SNPs (rs411021, rs436045, rs427811, rs385039 and rs809912) on gene expression and further identify the susceptibility genes of type 2 diabetes. METHODS: Ten allele fragments (49 bp each) were synthesized according to the 5 SNPs mentioned above. These fragments were cloned into luciferase reporter gene vector and then transfected into HepG2 cells. The activity of the luciferase was assayed. Effects of the SNPs on RNA splicing were analyzed by bioinformatics. RESULTS: rs427811T allele and rs809912G allele enhanced the activity of the reporter gene expression. None of the 5 SNPs affected RNA splicing. CONCLUSION: SNPs in protein kinase Cz (PKCZ) gene probably play a role in the susceptibility to type 2 diabetes by affecting the expression level of the relevant genes.
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页码：2357 / 2360
页数：4
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