High-throughput screening for biocatalysts

被引:122
|
作者
Wahler, D [1 ]
Reymond, JL [1 ]
机构
[1] Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland
关键词
D O I
10.1016/S0958-1669(01)00260-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Recent progress in high-throughput enzyme assays includes new examples of fluorogenic and chromogenic substrates, fluorescence resonance energy transfer substrates, and applications of the pH and pM indicator methods. Recent developments of Horeau's pseudo-enantiomer derivatisation method to screen enantioselectivities in high-throughput have also been reported.
引用
收藏
页码:535 / 544
页数:10
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