Expression of c-lysozyme Gene in Transgenic Potatoes cv. Jala Ipam Against Bacterial Wilt Disease Caused by Ralstonia solanacearum

The objective of this study was to analyze the expression of the c-lysozyme gene in transgenic potatoes cv. Jala Ipam and their resistance to bacterial wilt disease cultivated in the isolated field. Inoculation of Ralstonia solanacearum Race 3 onto transgenic (JCL2 and JCL3) and non-transgenic (NT) plants derived from GO tuber showed that all transgenic potatoes were more resistant to R. solanacearum than non-transgenic ones. JCL2 line was the most resistant line with disease frequency only 8.33%. Tuber production of JCL2 was 4 fold and JCL3 was 3.5 fold higher than non-transgenic line. The expression of c-lysozyme in transgenic lines was higher than that in non-transgenic line. JCL2 had the highest expression of the c-lysozyme gene compared to other lines. The relative expression of lysozyme gene in JCL2 was 4.83 fold to NT and 2.34 fold to JCL3. There was no expression of the c-lysozyme gene in non-transgenic line. It was found that higher expression of the c-lysozyme gene was correlated to higher resistance to bacterial wilt disease and to the higher tuber production. (C) 2020 Friends Science Publishers