Isolation of the murI gene from Brevibacterium lactofermentum ATCC 13869 encoding D-glutamate racemase

被引：6
|
作者：
Malathi, KC [1 ]
Wachi, M [1 ]
Nagai, K [1 ]
机构：
[1] Tokyo Inst Technol, Dept Bioengn, Midori Ku, Yokohama, Kanagawa 2268501, Japan
来源：
FEMS MICROBIOLOGY LETTERS | 1999年 / 175卷 / 02期
关键词：
coryneform bacterium; Brevibacterium lactofermentum; Corynebacterium glutamicum; D-glutamate racemase; murI;
D O I：
10.1016/S0378-1097(99)00192-5
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The I,murI gene encoding D-glutamate racemase plays an important role in the biosynthesis of D-glutamic acid, an essential component of cell wall peptidoglycan of almost all eubacteria. A DNA fragment that could rescue the auxotrophy of D-glutamic acid in the Escherichia coli murI mutant strain WM335 was isolated from Brevibacterium,? lactofermentum ATCC 13869 belonging to the coryneform bacteria. DNA sequencing reveals that it encodes a protein of 284 amino acid residues, which shows a high level of homology with D-glutamate racemases From several other bacteria. (C) 1999 Published by Elsevier Science B.V. All rights reserved.
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收藏
页码：193 / 196
页数：4
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