Alkali pretreatment method of dairy wastewater based grown Arthrospira platensis for enzymatic degradation and bioethanol production

Owing to increasing gasoline demand and fossil fuel depletion. Biofuel is increasingly being produced using an alternative source, such as algae. Microalgae is one such promising biofuel-generating solution. This investiga-tion used the Response Surface Methodology (RSM), focusing on Central Composite Design to improve the chemical pretreatment conditions for the microalgae (Arthrospira platensis). The significant influence of sodium hydroxide (NaOH) concentration and pretreated time on sugar yield and pretreatment processes were optimized using CCD. Pretreatment parameters such as duration (1 to 3 days) and NaOH concentrations (1 to 3 % (w/v)) accumulated in the pretreatment model. Following the pretreatment stages, the microalgae biomass was digested with a cellulase enzyme and Trichoderma sp. under three conditions cellulase enzyme 2 %, Trichoderma sp. 2 %, and cellulase enzyme 1 % combined with Trichoderma sp. 1 %. The combinations were incubated at 30 ?C for 1, 2, and 3 days, transferring to fermentable sugar for Saccharomyces cerevisiae TISTR5020 to use in bioethanol fermentation.