New technologies in affinity assays to explore biological communication

被引：10
|
作者：
Roper, Michael G. [1 ]
Guillo, Christelle [1 ]
机构：
[1] Florida State Univ, Dept Chem & Biochem, Tallahassee, FL 32306 USA
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2009年 / 393卷 / 02期
关键词：
Bioanalytical methods; Immunoassays/ELISA; PCR; Microfluidics/microfabrication; Capillary electrophoresis; Affinity; PLASMA-MASS SPECTROMETRY; CAPILLARY-ELECTROPHORESIS; IMMUNO-PCR; HIGH-SENSITIVITY; PROTEIN; INSULIN; LANGERHANS; SECRETION; GLUCAGON; SYSTEMS;
D O I：
10.1007/s00216-008-2347-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The language that cells use to communicate consists of the small molecules, peptides, and proteins that are released into the extracellular environment. To decipher this language, analytical assays are needed that have high selectivity, high sensitivity, and fast temporal resolution. Affinity assays are a group of analytical methodologies that are adept at studying this communication. In this overview, we highlight several examples from the literature on various types of affinity assays used in different platforms to monitor biological communication of peptides and proteins.
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页码：459 / 465
页数：7
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