Intercellular Bioimaging and Biodistribution of Gold Nanoparticle-Loaded Macrophages for Targeted Drug Delivery

被引:13
|
作者
Kim, Sehwan [1 ]
Kang, Sung Hun [2 ]
Byun, Soo Hwan [3 ]
Kim, Hye-Jin [4 ]
Park, In-Kyu [5 ]
Hirschberg, Henry [6 ,7 ]
Hong, Seok Jin [8 ]
机构
[1] Dankook Univ, Beckman Laser Inst Korea, Dept Biomed Engn, Coll Med, 119 Dandae Ro, Cheonan 31116, Chungnam, South Korea
[2] Hallym Univ, Dept Biomed Sci, Coll Med, Chunchon 24252, South Korea
[3] Hallym Univ, Dept Oral & Maxillofacial Surg, Dent, Coll Med, Anyang 14068, South Korea
[4] Tomocube Inc, 155 Sinseong Ro, Daejeon 34109, South Korea
[5] Chonnam Natl Univ, Dept Biomed Sci, Sch Med, Gwangju 61469, South Korea
[6] Univ Calif Irvine, Beckman Laser Inst, 1002 Hlth Sci Rd, Irvine, CA 92617 USA
[7] Univ Calif Irvine, Med Clin, 1002 Hlth Sci Rd, Irvine, CA 92617 USA
[8] Hallym Univ, Dept Otorhinolaryngol Head & Neck Surg, Coll Med, Dongtan Sacred Heart Hosp, 7 Keunjaebong Gil, Hwaseong Si 18450, Gyeonggi Do, South Korea
来源
ELECTRONICS | 2020年 / 9卷 / 07期
基金
新加坡国家研究基金会;
关键词
gold nanoparticle; macrophage; biodistribution; holotomography; drug delivery System; CANCER; INFLAMMATION; MIGRATION; THERAPIES;
D O I
10.3390/electronics9071105
中图分类号
TP [自动化技术、计算机技术];
学科分类号
0812 ;
摘要
In order to effectively apply nanoparticles to clinical use, macrophages have been used as vehicles to deliver genes, drugs or nanomaterials into tumors. In this study, the effectiveness of macrophage as a drug delivery system was validated by biodistribution imaging modalities at intercellular and ex vivo levels. We focused on biodistribution imaging, namely, the characterization of the gold nanoparticle-loaded macrophages using intracellular holotomography and target delivery efficiency analysis using ex vivo fluorescence imaging techniques. In more detail, gold nanoparticles (AuNPs) were prepared with trisodium citrate method and loaded into macrophage cells (RAW 264.7). First, AuNPs loading into macrophages was confirmed using the conventional ultraviolet-visible (UV-VIS) spectroscopy and inductively coupled plasma-mass spectrometry (ICP-MS). Then, the holotomographic imaging was employed to characterize the intracellular biodistribution of the AuNPs-loaded macrophages. The efficacy of target delivery of the well AuNPs uptake macrophages was studied in a mouse model, established via lipopolysaccharide (LPS)-induced inflammation. The fluorescent images and the ex vivo ICP-MS evaluated the delivery efficiency of the AuNPs-loaded macrophages. Results revealed that the holotomographic imaging techniques can be promising modalities to understand intracellular biodistribution and ex vivo fluorescence imaging can be useful to validate the target delivery efficacy of the AuNPs-loaded macrophages.
引用
收藏
页码:1 / 12
页数:12
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