Cloning, characterization, and sequence analysis of the clcE gene encoding the maleylacetate reductase of Pseudomonas sp. strain B13

被引：23
|
作者：
Kasberg, T [1 ]
Seibert, V [1 ]
Schlomann, M [1 ]
Reineke, W [1 ]
机构：
[1] BERG UNIV GESAMTHSCH WUPPERTAL,D-42097 WUPPERTAL,GERMANY
来源：
JOURNAL OF BACTERIOLOGY | 1997年 / 179卷 / 11期
关键词：
D O I：
10.1128/jb.179.11.3801-3803.1997
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A 3,167-bp PstI fragment of genomic DNA from Pseudomonas sp. strain B13 nas cloned and sequenced. The gene clcE consists of 1,059 nucleotides encoding a protein of 352 amino acids with a calculated mass of 37,769 Da which showed maleylacetate reductase activity. The protein had significant sequence similarities with the polypeptides encoded by tcbF of pP51 (59.4% identical positions), tfdF of pJP4 (55.1%), and tftE of Burkholderia cepacia AC1100 (53.1%). The function of TcbF as maleylacetate reductase was established by an enzyme assay.
引用
收藏
页码：3801 / 3803
页数：3
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