Sulfolobus solfataricus translation initiation factor 1 stimulates translation initiation complex formation

被引：22
|
作者：
Hasenöhrl, D
Benelli, D
Barbazza, A
Londei, P
Bläsi, U
机构：
[1] Univ Vienna, Vienna Bioctr, Dept Microbiol & Immunobiol, Max F Perutz Labs, A-1030 Vienna, Austria
[2] Univ Rome, Dept Cellular Biotechnol & Hematol, I-00161 Rome, Italy
[3] Univ Bari, Dept Med Biochem Biol & Phys, I-70124 Bari, Italy
来源：
RNA | 2006年 / 12卷 / 04期
关键词：
aIF1; archaea; Sulfolobus solfataricus; translation initiation;
D O I：
10.1261/rna.2289306
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The eukaryotic translation initiation factor 1 binds to the ribosome during translation initiation. It is instrumental for initiator-RNA and mRNA binding, and has a function in selection of the authentic start codon. Here, we show that the archaeal homolog aIF1 has analogous functions. The aIF1 protein of the archaeon Sulfolobus solfataricus is bound to the small ribosomal subunit during translation initiation and accelerates binding of initiator-tRNA and mRNA to the ribosome. Accordingly, aIF1 stimulated translation of an mRNA in a S. solfataricus in vitro translation system. Moreover, this study suggested that the C terminus of the factor is of relevance for its function.
引用
收藏
页码：674 / 682
页数：9
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