Transformation with green fluorescent protein of Trichoderma harzianum 1051, a strain with biocontrol activity against Crinipellis perniciosa, the agent of witches'-broom disease of cocoa

被引：7

作者：

Inglis, PW ^{[1
]}

Queiroz, PR ^{[1
]}

Valadares-Inglis, MC ^{[1
]}

机构：

[1] EMBRAPA, CENARGEN, Microbial Mol Genet Lab, LGM, BR-70770900 Brasilia, DF, Brazil

来源：

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | 1999年 / 45卷 / 02期

关键词：

biological control; cocoa; cotransformation; Crinipellis perniciosa; green fluorescent protein; Trichoderma harzianum; witches'-broom disease;

D O I：

10.2323/jgam.45.63

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A plasmid vector for fungal expression of an enhanced, red-shifted variant of the Aequoria victoriae green fluorescent protein was constructed by fusion of the EGFP gene to the highly expressed Aspergillus nidulans gpd promoter and the A. nidulans trpC terminator. This construction was introduced by cotransformation, using benomyl selection, into Trichoderma harzianum strain 1051, a strain being evaluated for the biological control of witches'-broom disease of cocoa caused by Crinipellis perniciosa. Epifluorescence microscopy was used to monitor germination and attachment of stable transformant conidia on the surface of C. perniciosa hyphae.

引用

页码：63 / 67

页数：5

共 16 条

[1] Characterization of an amylase produced by a Trichoderma harzianum isolate with antagonistic activity against Crinipellis perniciosa, the causal agent of witches' broom of cocoa
de Azevedo, AMC
De Marco, JL
Felix, CR
FEMS MICROBIOLOGY LETTERS, 2000, 188 (02) : 171 - 175
[2] A Trichoderma harzianum chitinase destroys the cell wall of the phytopathogen Crinipellis perniciosa, the causal agent of witches' broom disease of cocoa
Janice Lisboa De Marco
Luzia Helena C. Lima
Marcelo Valle de Sousa
Carlos R. Felix
World Journal of Microbiology and Biotechnology, 2000, 16 : 383 - 386
[3] A Trichoderma harzianum chitinase destroys the cell wall of the phytopathogen Crinipellis perniciosa, the causal agent of witches' broom disease of cocoa
De Marco, JL
Lima, LHC
de Sousa, MV
Felix, CR
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2000, 16 (04): : 383 - 386
[4] Production of hydrolytic enzymes by Trichoderma isolates with antagonistic activity against Crinipellis perniciosa, the causal agent of witches broom of cocoa
De Marco, JL
Valadares-Inglis, MC
Felix, CR
BRAZILIAN JOURNAL OF MICROBIOLOGY, 2003, 34 (01) : 33 - 38
[5] PLEOMORPHISM IN CRINIPELLIS-PERNICIOSA, CAUSAL AGENT OF WITCHES BROOM DISEASE OF COCOA
EVANS, HC
TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1980, 74 (JUN): : 515 - 523
[6] Development of a transformation system for Crinipellis perniciosa, the causal agent of witches' broom in cocoa plants
Lima, JO
dos Santos, JK
Pereira, JF
de Resende, MLV
de Araújo, EF
de Queiroz, MV
CURRENT GENETICS, 2003, 42 (04) : 236 - 240
[7] Development of a transformation system for Crinipellis perniciosa, the causal agent of witches' broom in cocoa plants
Juliana Oliveira Lima
Jildete Karla dos Santos
Jorge Fernando Pereira
Mário Lúcio Vilela de Resende
Elza Fernandes de Araújo
Marisa Vieira de Queiroz
Current Genetics, 2003, 42 : 236 - 240
[8] Development of a transformation system for Crinipellis perniciosa, the causal agent of witches' broom in cocoa plants
Juliana Oliveira Lima
Jildete Karla dos Santos
Jorge Fernando Pereira
Mário Lúcio Vilela de Resende
Elza Fernandes de Araújo
Marisa Vieira de Queiroz
Current Genetics, 2003, 43 : 70 - 70
[9] INVITRO PRODUCTION OF BASIDIOSPORES OF CRINIPELLIS-PERNICIOSA, THE CAUSATIVE AGENT OF WITCHES-BROOM DISEASE OF COCOA
BASTOS, CN
ANDEBRHAN, T
TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1987, 88 : 406 - 409
[10] Scanning electron microscopy of conidia of Trichoderma stromaticum, a biocontrol agent of witches' broom disease of cocoa
de Melo, IS
Faull, JL
BRAZILIAN JOURNAL OF MICROBIOLOGY, 2004, 35 (04) : 330 - 332

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