Low-intensity pulsed ultrasound-mediated stimulation of hematopoietic stem/progenitor cell viability, proliferation and differentiation in vitro

被引：42
|
作者：
Xu, Peng [1 ,2 ]
Gul-Uludag, Hilal [1 ,3 ,4 ]
Ang, Woon T. [4 ]
Yang, Xiaoyan [1 ]
Huang, Min [1 ]
Marquez-Curtis, Leah [5 ]
McGann, Locksley [5 ]
Janowska-Wieczorek, Anna [6 ]
Xing, James [4 ,7 ]
Swanson, Eric [8 ]
Chen, Jie [1 ,2 ,3 ]
机构：
[1] Univ Alberta, Dept Biomed Engn, ECERF W6 019, Edmonton, AB T6G 2V4, Canada
[2] Natl Inst Nanotechnol, Edmonton, AB T6G 2M9, Canada
[3] Univ Alberta, Dept Elect & Comp Engn, Edmonton, AB T6G 2V4, Canada
[4] IntelligentNano Inc, Edmonton, AB T6G 2M9, Canada
[5] Canadian Blood Serv R&D, Edmonton, AB T6G 2R8, Canada
[6] Univ Alberta, Dept Med, Edmonton, AB T6G 2R8, Canada
[7] Cross Canc Inst, Edmonton, AB T6G 1Z2, Canada
[8] Natl Res Council Canada IRAP, Edmonton, AB T6N 1E4, Canada
来源：
BIOTECHNOLOGY LETTERS | 2012年 / 34卷 / 10期
基金：
加拿大自然科学与工程研究理事会; 加拿大健康研究院;
关键词：
CD34(+) cells; Cord blood; Low-intensity pulsed ultrasound; Peripheral blood leukapheresis product; Stem cell therapy; STEM-CELL; THERAPEUTIC ULTRASOUND; FIBROBLASTS; EXPRESSION; GROWTH;
D O I：
10.1007/s10529-012-0984-6
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Low-intensity pulsed ultrasound (LIPUS) stimulated the viability, proliferation and differentiation of hematopoietic stem/progenitor cells (HSPC) from fresh and cryopreserved peripheral blood leukapheresis product, as well as cord blood when applied for 10 min each day for 4 days. Cell viability, proliferation and differentiation were assessed on day 5 by viable cell counting, MTS proliferation assay, flow cytometry, and colony-forming unit assay. LIPUS stimulation: (i) enhanced the proliferation of fresh HSPC and maintained the viability of cryopreserved HSPC in vitro; (ii) did not affect the percentage of CD34(+) and CD14(+) cells; and (iii) enhanced burst-forming unit-erythroid colony formation. Hence, we suggest that this novel LIPUS stimulation approach might enhance the efficacy of clinical transplantation and cellular therapies using HSPC.
引用
收藏
页码：1965 / 1973
页数：9
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