Leishmania (Viannia) braziliensis: New primers for identification using polymerase chain reaction

被引：13
|
作者：
Marcussi, V. M. [2 ]
Marcussi, L. M. [2 ]
Barbosa-Tessmann, I. P. [3 ]
Lonardoni, M. V. C. [1 ]
Silveira, T. G. V. [1 ]
机构：
[1] Univ Estadual Maringa, Dept Clin Anal, BR-87020900 Maringa, Parana, Brazil
[2] Univ Estadual Maringa, Master Program Clin Anal, BR-87020900 Maringa, Parana, Brazil
[3] Univ Estadual Maringa, Dept Biochem, BR-87020900 Maringa, Parana, Brazil
来源：
EXPERIMENTAL PARASITOLOGY | 2008年 / 120卷 / 04期
关键词：
Leishmaniasis; Leishmania braziliensis; DNA primers; Polymerase chain reaction;
D O I：
10.1016/j.exppara.2008.08.005
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The objective of this study was to develop specific primers for Leishmania (Viannia) braziliensis species identification using PCR. The designed primers (LBF1 and LBR1) were evaluated for sensitivity and specificity using various L. (V.) braziliensis serodemes and various Leishmania species and also using Trypanosoma cruzi. A specific fragment of 536 bp was detected from 50 ng of DNA in a crude extract derived from L. (V.) braziliensis. The DNA fragment was not detected when DNA from other Leishmania species or from T cruzi was used as template in the PCR. Furthermore, when tested with DNA from cutaneous leishmaniasis the designed primers and reaction gave positive results. Taking into consideration that the primers LBF1 and LBR1 could specifically identify L. (V.) braziliensis, they could be considered for use in L. (V.) braziliensis diagnosis and epidemiological studies. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：300 / 305
页数：6
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