The complete mitochondrial genome ofHomatula pycnolepis(Teleostei, Cypriniformes) in Lantsang River and its phylogenetic analysis

Homatula pycnolepisis a small-sized benthopelagic fish species in the family Nemacheilidae, superfamily Cobitidea. In this study, the entire mitogenome sequence of theH. pycnolepishas been sequenced by using sanger sequencing method. The circular mitochondrial genome was 16,570 bp in length and contained of 13 protein-coding genes, two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, an origin of light-strand replication (OL), and one displacement loop locus (D-loop). The overall base composition was 30.1% A, 26.4% T, 26.8% C, and 16.7% G with 56.6% AT content. Most genes were encoded on the heavy strand except for ND6 and eight tRNA genes. There were 11 regions of gene overlaps totaling 29 bp and 7 intergenic spacer regions totaling 37 bp. Its structure type was similar to the mitogenome of Cobitidea fishes. Phylogenetic analysis with 33 complete mitogenomes of Cobitidea and2 Macrognathusfishes in the family of Mastacembelidae suggested thatHomatulaspecies clustered as one monophyletic clade andHomatulawas closely related toSchistura.Mitogenome information from this study could be a useful basis for conservation and phylogenetics of this species.