Optimization and microbial diversity of anaerobic co-digestion of swine manure with waste kitchen oil at high organic loading rates

Anaerobic co-digestion of swine manure (SM) and waste kitchen oil (WKO) was conducted to evaluate the effect of high organic loading rates (OLRs) on biogas production efficiency and microbial changes. Combinations of different loading rates of SM and WKO, with total OLRs from 2 to 8 g VS (volatile solid)/L/d, were evaluated in a laboratory-scale study. While feeding more than 4 g VSSM/L/d did not result in higher biogas production in both mono-and co-digestion scenarios, the addition of WKO increased the total OLR up to 6 g VS/L/d without sig-nificant reduction of system productivity. Biogas yields of M2O1 (2 g VSSM/L/d + 1 g VSWKO/L/d) and M4O2 were 910 +/- 35 and 849 +/- 85 mL/g VSfed which were 25.2 % and 16.9 % higher than the mono-digestion of M2, respectively. A significant increase of bacterial alpha-diversity (Shannon index) was observed in M2O1, at 233.0 +/- 3.6 compared with 218.7 +/- 5.1 of M2 (p < 0.05). Less bacterial alpha-diversity and accumulation of volatile fatty acids were observed in M4O1 and M4O2, suggesting their potential instability. When digesters were fed with M2, the introduction of 1.4 g VSWKO/L/d or more did not increase biogas yield and could cause system imbalance. The study suggests the limit of WKO could be increased when higher OLRs of SM were applied but should not be more than 4 g VSSM/L/d, and ratio between SM and WKO should be considered to avoid failure. Some of the system disturbances took up to three months to show.