Glucose-induced changes in the redox state of pancreatic non-B islet cells

被引:0
|
作者
Mercan, D [1 ]
Malaisse, WJ [1 ]
机构
[1] FREE UNIV BRUSSELS,ERASMUS SCH MED,EXPTL MED LAB,B-1070 BRUSSELS,BELGIUM
来源
MEDICAL SCIENCE RESEARCH | 1996年 / 24卷 / 01期
关键词
pancreatic islet cells; flow cytometry; redox state;
D O I
暂无
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Pancreatic islet B and non-B cells prelabelled with the calcium-sensitive fluorochrome Fluo-3 were examined in a single step procedure of flow cytometry. Increasing concentrations of D-glucose, in the 1.0-20.0 mM range, were found to cause a progressive rise in the NAD(P)H content of the islet non-B cells. This coincided with a dual change in the intracellular Ca2+ concentration in the same cells. In the non-B cells, the forward scatter of light was little affected, however, by changes in D-glucose concentration. These findings are compared with those found, within the same experiments, in purified islet B-cells, with the emphasis on the differences between the two cell populations in their responsiveness to D-glucose, in terms of changes in both redox state and Ca2+ cell content.
引用
收藏
页码:31 / 33
页数:3
相关论文
共 50 条
  • [1] GLUCOSE-INDUCED ELECTRICAL ACTIVITY IN PANCREATIC ISLET CELLS
    DEAN, PM
    MATTHEWS, EK
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1970, 210 (02): : 255 - &
  • [2] ALLOXAN UPTAKE BY PANCREATIC B AND NON-B ISLET CELLS
    MALAISSE, WJ
    GORUS, FK
    PIPELEERS, DG
    [J]. BIOLOGY OF THE CELL, 1982, 45 : 282 - 282
  • [3] Glucose-induced changes in mitochondrial redox status in rat pancreatic beta cells
    Roma, L. P.
    Duprez, J.
    Takahashi, H. K.
    Wiederkehr, A.
    Jonas, J. -C.
    [J]. DIABETOLOGIA, 2011, 54 : S191 - S191
  • [5] The coupling of metabolic to secretory events in pancreatic islets. Glucose-induced changes in mitochondrial redox state
    Ramirez, R
    Rasschaert, J
    Sener, A
    Malaisse, WJ
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1996, 1273 (03): : 263 - 267
  • [6] Effects of D-mannoheptulose upon D-glucose metabolism in pancreatic B and non-B islet cells
    Ramirez, R
    Jijakli, H
    Zhang, HX
    Nadi, AB
    Sener, A
    Malaisse, WJ
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2002, 9 (02) : 159 - 163
  • [7] PURIFICATION OF PANCREATIC B AND NON-B ISLET CELLS LOADED WITH A FLUORESCENT CALCIUM INDICATOR
    MERCAN, D
    CONGET, I
    LECLERCQMEYER, V
    MALAISSE, WJ
    [J]. DIABETOLOGIA, 1994, 37 : A100 - A100
  • [8] PANCREATIC ACINAR AND ISLET CELLS IN COCULTURE - SELECTIVITY OF NON-B ENDOCRINE-CELLS
    ARIAS, AE
    BENDAYAN, M
    [J]. EUROPEAN JOURNAL OF CELL BIOLOGY, 1993, 61 (01) : 193 - 196
  • [9] Regulatory Role of Fatty Acid Metabolism on Glucose-Induced Changes in Insulin and Glucagon Secretion by Pancreatic Islet Cells
    Tamarit-Rodriguez, Jorge
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (11)
  • [10] Glucose-induced changes in the redox status of retinal endothelial cells and pericytes
    Gao, L
    Chibber, R
    Siow, R
    Mann, G
    [J]. FASEB JOURNAL, 2005, 19 (05): : A1232 - A1233