Isolation and Identification of Photosynthetic Bacteria and Their Hydrogen Production Characterization

Using special culture medium, dominant photosynthetic bacterias were enriched from sewage silt and paddy fields with plentiful light and organic compounds. Five strains of photosynthetic bacteria capable of producing hydrogen were purified and isolated by gradual dilution and double-deck solid medium. The analysis of the gene sequence information of 1 6SrDNA was carried out, and the five strains were identified as Rhodospirillum rubrum,R.capsulata, R.pulastris, Rhodobacter capsulatus, Rhodobacter capsulatus . Moreover, it was found that with Optimized medium formula, hydrogen production can be achieved 204h, the maximum hydrogen production capacity of 3.41L, the maximum hydrogen production rate was 44.17ml / (L . h),the highest hydrogen content of 46.73%.